Phylum Firmicutes, Class Bacilli, Order Bacillales, Family Bacillaceae, Genus Bacillus, Solibacillus, Solibacillus silvestris (Rheims,
Frühling, Schumann, Rohde and Stackebrandt 1999) Krishnamurthi, Chakrabarti and Stackebrandt 2009.
Basonym: Bacillus silvestri Rheims, Frühling, Schumann, Rohde and Stackebrandt 1999.
Description is based upon a single isolate.
Cell-wall composition (cell-wall peptidoglycan contains lysine, glutamic acid and
alanine) differentiates this species from Lysinibacillus fusiformis, L.sphaericus and
Gram-positive rods, 0.5-0.7 x 0.9-2.0 µm, motile by peritrichous flagella. Produce
terminally, spherical endospores in swollen sporangia.
Colonies are whitish and shiny. Grow in TSB and TSA. Aerobic. Growth temperature
10-40 ºC (optimum 20-30 ºC); pH 7, 0-5% NaCl. Alantoin or urate are not required for
Isolated from the top soil layer of a beech forest soil near Braunschweig, Lower Saxony, Germany. No growth in the presence of
- N.A. Logan and P. De Vos, 2009. Genus I. Bacillus Cohn 1872. In: (Eds.) P.D. Vos, G. Garrity, D. Jones, N.R. Krieg, W. Ludwig, F.
A. Rainey, K.-H. Schleifer, W.B. Whitman. Bergey’s Manual of Systematic Bacteriology, Volume 3: The Firmicutes, Springer, 21-127.
- H. Rheims, A. Frühling, P. Schumann, M. Rohde and E. Stackebrandt, 1999. Bacillus silvestris sp. nov., a new member of the
genus Bacillus that contains lysine in its cell wall. IJSB 49, 795-802.
- S. Krishnamurthi, T. Chakrabarti and E. Stackebrandt, 2009. Re-examination of the taxonomic position of Bacillus silvestris
Rheims et al. 1999 and proposal to transfer it to Solibacillus gen. nov. as Solibacillus silvestris comb. nov. IJSEM 59, 1054-1058.
Positive results for catalase.
Negative results for arginine dihydrolase, deamination of phenylalanine, hydrolysis of esculin, hydrolysis of hippurate, hydrolysis of
gelatin, egg yolk reaction, oxidase, indole, nitrate reduction, hydrolysis of casein, hydrolysis of starch, Voges-Proskauer test, citrate
utilization, hydrolysis of Tween 80, degradation of tyrosine, acid production from: L- or D-arabinose, N-acetyl-D-glucosamine, adonitol,
amygdalin, L- or D-arabitol, arbutin, cellobiose, dulcitol, erythritol, fructose, D-fucose, galactose, beta-gentibiose, gluconate,
D-glucose, glycerol, glycogen, meso-inositol, inulin, 2- or 5-ketogluconate, lactose, lyxose, D-mannitol, D-mannose, methyl
beta-xyloside, maltose, melezitose, melibiose, raffinose, rhamnose, ribose, sorbitol, sorbose, sucrose, salicin, starch, trehalose,
xylitol, L- or D-xylose.
(c) Costin Stoica