Taxonomy
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Phylum Firmicutes, Class Bacilli, Order Bacillales, Family Bacillaceae, Genus Bacillus, Bacillus siamensis  Sumpavapol, Tongyonk,
Tanasupawat, Chokesajjawatee, Luxananil and Visessanguan 2010.
Description is based on a single isolate.
Gram-positive, motile (peritrichous flagella) rods, 0.3-0.6 x 1.5-3.5 µm. Produce
central / subterminal, ellipsoidal endospores  in swollen sporangia.
Colonies are creamy white, mucoid, translucent, raised, entire margins, 3-4 mm in
diameter after 2 days at 37 ºC on TSA; in liquid medium, a thin film is formed at the
surface whilst the rest of the medium is uniformly cloud.
Grows on MacConkey agar, not on bile salt medium.
Aerobic, facultatively anaerobic. Growth temperature 4-55 ºC (optimum 37 ºC), pH
4.5-9 (optimum pH  6-7). Grow in 0-14% NaCl (optimum 0% NaCl).
Isolated from salted crab (poo-khem) in Thailand.
Undetermined.
  1. Sumpavapol P., Tongyonk L., Tanasupawat S., Chokesajjawatee N., Luxananil P. and Visessanguan W., 2010. Bacillus siamensis
    sp. nov., isolated from salted crab (poo-khem) in Thailand. IJSEM 60, 2364-2370.
Positive results for catalase, nitrate reduction, hydrolysis of casein, hydrolysis of starch, Voges-Proskauer, alkaline phosphatase,  
esterase, esterase lipase, hydrolysis of esculin, hydrolysis of gelatin, acid production from: L-arabinose, amygdalin, arbutin,
cellobiose, fructose, beta-gentiobiose, D-glucose, glycerol, glycogen, meso-inositol, lactose, maltose, D-mannitol, raffinose, ribose,
sorbitol, salicin, starch, sucrose & D-xylose.

Negative results for degradation of tyrosine, deamination of phenylalanine, beta-galactosidase, hydrolysis of urea, oxidase,  indole
production, H
2S production, citrate utilization, lipase, hydrolysis of Tween 80, tryptophan deaminase, methyl-red, trypsin, acid
phosphatase, acid production from: adonitol, D-arabinose, D- or L-arabitol, dulcitol, erythritol, D- or L-fucose, galactose, gluconate,
N-acetyl-D-glucosamine, inulin, 2- or 5-ketogluconate, lyxose, D-mannose, melezitose, melibiose, rhamnose, sorbose, trehalose,
xylitol & L-xylose.
Bacillus siamensis
(c) Costin Stoica
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