Phylum Firmicutes, Class Bacilli, Order Bacillales, Family Bacillaceae, Genus Bacillus, Bacillus safensis Satomi, La Duc, and
Venkateswaran 2006. Biochemically and by 16S rRNA gene sequencing is very similar to Bacillus pumilus.
Gram-positive, motile by polar flagella, spore-forming rods, 0.5-0.7 x 1.0-1.2 µm.
Colonies are undulate, with irregular margins, dull white on TSA in 24 h at 32 ºC.
Grow at 10-50 ºC (optimum growth at 30-37 ºC) and in media containing 0-10% NaCl.
Aerobic growth; no anaerobic growth. Chemoheterotrophic. Growth does not occur in
0.0001% lysozyme broth.
Isolated from the spacecraft surface and assembly facility floor of the Mars Odyssey and from the spacecraft-assembly facility of the
Jet Propulsion Laboratory, Pasadena, CA, USA.
- M. Satomi, M.T. La Duc and K. Venkateswaran 2006. Bacillus safensis sp. nov., isolated from spacecraft and assembly-facility
surfaces. IJSEM 56, 1735-1740.
Positive results for oxidase, catalase, beta-galactosidase, hydrolysis of esculin,
hydrolysis of gelatin, Voges-Proskauer test, acid production from: L-arabinose,
amygdalin, arbutin, cellobiose, fructose, galactose, D-glucose (without gas), glycerol,
N-acetyl-D-glucosamine, meso-inositol, maltose, D-mannitol, D-mannose, ribose,
salicin, sucrose, trehalose, D-turanose & D-xylose.
Negative results for arginine dihydrolase, lysine decarboxylase, ornithine decarboxylase, deamination of phenylalanine, hydrolysis of
urea, indole production, H2S prodction, nitrate reduction, citrate utilization, tryptophan deaminase, amylase, acid production from:
adonitol, D-arabinose, D- or L-arabitol, dulcitol, erythritol, D- or L-fucose, beta-gentiobiose, gluconate, glycogen, inulin, 2- or
5-ketogluconate, lyxose, melezitose, raffinose, rhamnose, sorbitol, sorbose, starch, xylitol & L-xylose.
Variable results for hydrolysis of casein, acid production from lactose, gentibiose & melibiose.
Can be differentiated from B. pumilus by acid production from inositol, methyl alpha-D-glucopyranoside, maltose, D-turanose,
utilization of inositol, and negative results for lipase (Tween 80) & casein hydrolysis in litmus milk.
(c) Costin Stoica