Phylum Firmicutes, Class Bacilli, Order Bacillales, Family Bacillaceae, Genus Psychrobacillus, Psychrobacillus psychrotolerans (Abd
El-Rahman, Fritze, Sproer and Claus 2002) Krishnamurthi, Ruckmani, Pukall and Chakrabarti 2010.
Phenotypically very closed to Psychrobacillus insolitus and Psychrobacillus psychrodurans, and separable from these species by
anaerobic growth with KNO3, nitrate reduction and NaCl tolerance.
Basonym: Bacillus psychrotolerans Abd El-Rahman, Fritze, Sproer and Claus 2002.
Gram-positive, non-motile rods, 0.4-1.0 x 2.0-7.0 µm. Sporulation infrequently
observed; terminally endospores in swollen sporangia. Spores are predominantly
spherical in casein-peptone soymeal-peptone agar cultures and predominantly
ellipsoidal in marine agar cultures.
Growth temperature -2 to 40 ºC, 2-5% NaCl (variable) and pH 7. No growth or very
poor growth on nutrient agar or in nutrient broth. Aerobic, no growth anaerobically or in
media containing KNO3. Allantoin or urate are not required for growth.
Isolated from field soil in Germany. No growth in the presence of lysozyme.
- N.A. Logan and P. De Vos, 2009. Genus I. Bacillus Cohn 1872. In: (Eds.) P.D. Vos, G. Garrity, D. Jones, N.R. Krieg, W. Ludwig, F.A.
Rainey, K.-H. Schleifer, W.B. Whitman. Bergey’s Manual of Systematic Bacteriology, Volume 3: The Firmicutes, Springer, 21-127.
- H. A. Abd El-Rahman, D. Fritze, C. Sproer and D. Claus, 2002. Two novel psychrotolerant species, Bacillus psychrotolerans sp.
nov. and Bacillus psychrodurans sp. nov., wich contain ornithine in their cell walls. IJSEM 52, 2127-2133.
Positive for oxidase, catalase, hydrolysis of starch, hydrolysis of Tween 20, 40 & 60.
Negative results for nitrate reduction, degradation of tyrosine, deamination of phenylalanine, hydrolysis of esculin, urease, egg yolk
reaction, indole production, hydrolysis of casein, Voges-Proskauer reaction & citrate utilization.
Variable or weak results for gelatinase, hydrolysis of Tween 80, acid production from: L-arabinose, D-glucose, D-mannitol & D-xylose.
(c) Costin Stoica