Taxonomy
Morphology
Cultural characteristics
Biochemical characters
Ecology
Pathogenicity
References
Phylum Firmicutes, Class Bacilli, Order Bacillales, Family Bacillaceae, Genus Bacillus, Bacillus pseudalcaliphilus corrig. Nielsen et
al. 1995 (original name:
Bacillus pseudalcalophilus).
It is phenotypically closely related to
Bacillus alcalophilus but phylogenetically distinct.
Rod shaped, 0.5-0.6 x 2.0-4.0 µm, producing paracentral / subterminal, ellipsoidal
endospores (0.5-0.7 x 0.8-1.3 µm) in swollen sporangia.
Colonies are white with undulate margins. Grow aerobically (anaerobic growth was
not tested). Alkaliphilic. Growth temperature 10-40 ºC (optimum 30 ºC); pH 8-10
(optimum pH 10). Can grow in 5-10% NaCl. NaCl, alantoin or urate are not required
for growth.
Isolated from soil.
Undetermined.
  1. N.A. Logan and P. De Vos, 2009. Genus I. Bacillus Cohn 1872. In: (Eds.) P.D. Vos, G. Garrity, D. Jones, N.R. Krieg, W. Ludwig, F.A.
    Rainey, K.-H. Schleifer, W.B. Whitman. Bergey’s Manual of Systematic Bacteriology, Volume 3: The Firmicutes, Springer, 21-127.
  2. P. Nielsen, D. Fritze and F.G. Priest, 1995. Phenetic diversity of alkaliphilic Bacillus strains: proposal for nine new species.
    Microbiology 141, 1745-1761.
Positive results for hydrolysis of gelatin, casein & hydrolysis of starch.

Negative results for deamination of phenylalanine, hydrolysis of hippurate, nitrate
reduction, hydrolysis of Tween 20 & Tween 80. No
growth in Voges-Proskauer test and in acid production tests from: L-arabinose, D-glucose, D-mannitol, D-mannose, methyl
beta-xyloside, glycogen, salicin, starch & D-xylose.

Variable results for hydrolysis of Tween 40 & Tween 60.
Bacillus pseudalcaliphilus
(c) Costin Stoica
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