Phylum Firmicutes, Class Bacilli, Order Bacillales, Family Bacillaceae, Genus Bacillus, Bacillus polygoni Aino, Hirota,Matsuno,
Morita, Nodasaka, Fujiwarw, Matsuyama, Yoshimune and Yumoto 2008.
Description of a single isolate.
Gram positive, straight rods, 0.4-0.5 x 1.0-3.5 µm. Produce central, ellipsoidal
endospores in unswollen sporangia. Nonmotile.
Colonies are white, circular when grown on PYA medium. Moderately halophilic and
obligately alkaliphilic. Grow at pH 8-12 (optimum pH 9), in 3-14% NaCl media
(optimum 7%) and at 13-47 ºC (optimum 30 ºC). NaCl is required for growth. Aerobic;
no anaerobic growth.
Isolated from indigo balls (fermentation of Polygonum tinctorium / Persicaria tinctoria), Ibaraki, Japan.
- Aino K., Hirota K., Matsuno T., Morita N., Nodasaka Y., Fujiwara T., Matsuyama H., Yoshimune K. and Yumoto I., 2008. Bacillus
polygoni sp. nov., a moderately halophilic, non-motile obligate alkaliphile isolated from indigo balls. IJSEM 58, 120-124.
Positive results for catalase, nitrate reduction, beta-galactosidase, hydrolysis of
casein, hydrolysis of Tween 20, 40, 60 & 80, acid production (when grown at pH 10) from: D-arabinose, fructose, D-mannitol,
D-mannose, glycerol, D-glucose, myo-inositol, maltose, raffinose, sucrose & D-xylose.
Negative results for oxidase, indole production H2S production, deamination of phenylalanine, hydrolysis of esculin, hydrolysis of
hippurate, hydrolysis of gelatin, hydrolysis of starch, hydrolysis of cellulose, hydrolysis of xylan, acid production (when grown at pH 10)
from: erythritol, galactose, lactose, melibiose, rhamnose, sorbitol, salicin & xylitol.
(c) Costin Stoica