Phylum Firmicutes, Class Bacilli, Order Bacillales, Family Bacillaceae, Genus Bacillus, Bacillus okuhidensis Li, Kawamura, Shida,
Yamagata, Deguchi and Ezaki 2002.
Description is based upon two isolates.
Weakly Gram-positive, motile rods (peritrichous flagella), 0.5-1.0 x 5.0-7.0 µm. Cells
stain slightly Gram-positive in the exponential growth phase and Gram-negative in the
stationary phase. Produce subterminal, ellipsoidal endospores in sometime slightly
Colonies are convex, smooth, yellowish. Aerobic. Alkaliphilic. Growth temperature
30-60 ºC (optimum 50 ºC); pH 6-11 (optimum pH 10.5). Grow in 0-10% NaCl. Alantoin
or urate are not required for growth.
Isolated from hot spa water, in Okuhida, Gifu, Japan.
- N.A. Logan and P. De Vos, 2009. Genus I. Bacillus Cohn 1872. In: (Eds.) P.D. Vos, G. Garrity, D. Jones, N.R. Krieg, W. Ludwig, F.A.
Rainey, K.-H. Schleifer, W.B. Whitman. Bergey’s Manual of Systematic Bacteriology, Volume 3: The Firmicutes, Springer, 21-127.
- Li, Y. Kawamura, O. Shida, S. Yamagata, T. Deguchi and T. Ezaki, 2002. Bacillus okuhidensis sp. nov., isolated from the Okuhida
spa area of Japan. JSEM 52, 1205-1209.
Positive results for hydrolysis of gelatin, oxidase, catalase, nitrate reduction, hydrolysis of casein & hydrolysis of starch.
Negative results for deamination of phenylalanine, hydrolysis of hippurate, hydrolysis of Tween 20, Tween 40 & Tween 80.
(c) Costin Stoica