Phylum Firmicutes, Class Bacilli, Order Bacillales, Family Bacillaceae, Genus Bacillus, Bacillus niacini Nagel and Andreesen 1991.
Rods, 0.9-1.4 x 3.0-5.6 µm; cells may be pleomorphic and increase in width. Long
chains may be formed when grown on complex media. Gram-positive on nicotinate
agar and Gram-variable in nutrient broth. Motility is variable. Produce central or
subterminal, ellipsoidal endospores wich may swell the sporangia slightly.
Sporulation was poor in complex media, satisfactory on nicotinate agar and it was
good on phenol red agar containing organic acid (acetate, aspartate, glutamate, and
Colonies are smooth, with light beige center surrounded by translucent areas of
variable extension, 3-5 mm in diameter. Aerobic. Growth in anaerobic conditions is
variable or weak. Growth temperature 10-40 ºC; pH 7-8 (optimum). No growth in 2-8%
NaCl medium. NaCl, alantoin or urate are not required for growth.
Nicotinate, in the presence of molybdate, can be used as sole source of carbon,
nitrogen and energy
Isolated from soil. Growth in the presence of lysozyme (0.001%) is variable.
- N.A. Logan and P. De Vos, 2009. Genus I. Bacillus Cohn 1872. In: (Eds.) P.D. Vos, G. Garrity, D. Jones, N.R. Krieg, W. Ludwig, F.
A. Rainey, K.-H. Schleifer, W.B. Whitman. Bergey’s Manual of Systematic Bacteriology, Volume 3: The Firmicutes, Springer, 21-127.
- M. Nagel and J.R. Andreesen, 1991. Bacillus niacini sp. nov., a Nicotinate-Metabolizing Mesophile Isolated from Soil. IJSB 41, 1,
Positive results for catalase (weak), beta-galactosidase, hydrolysis of gelatin (weak), nitrate reduction, acid production from:
N-acetyl-D-glucosamine, amygdalin, fructose, D-glucose, galactose, D-mannose, maltose, starch, sucrose, trehalose, L- or D-xylose.
Negative results for arginine dihydrolase, degradation of tyrosine, deamination of phenylalanine, lysine decarboxylase, ornithine
decarboxylase, egg yolk reaction, hydrolysis of casein, Voges-Proskauer reaction, acid production from: L- or D-arabinose, L- or
D-arabitol, adonitol, erythritol, L- or D-fucose, gluconate, D-mannitol, 2- or 5-ketogluconate & methyl beta-xyloside.
Variable results for hydrolysis of urea, indole production, citrate utilization, oxidase, hydrolysis of starch, acid production from: arbutin,
cellobiose, dulcitol, glycerol, glycogen, beta-gentibiose, lactose, melezitose, melibiose, meso-inositol, inulin, raffinose, rhamnose,
ribose, sorbitol, salicin & xylitol.
(c) Costin Stoica