Phylum Firmicutes, Class Bacilli, Order Bacillales, Family Paenibacillaceae, Genus Paenibacillus, Paenibacillus nanensis
Khianngam, Akaracharanya, Tanasupawat, Lee and Lee, 2009.
Gram-positive, motile (peritrichous flagella) rods, 1-1.2 x 3-8 µm. Produce ellipsoidal,
central / subterminal spore in swollen sporangium.
Colonies are white, convex, 1-3 mm in diameter after incubation at 37 ºC 2 days on C
agar (5 g peptone Difco, 1 g yeast extract Difco, 4 g K2HPO4, 1 g MgSO4 * 7H2O, 0.2 g
KCl,0.02 g FeSO4 * 7H2O and 1000 ml distilled water and adjusted to pH 7.0). Aerobic
and anaerobic growth. Growth temperature 20-45 ºC, optimum 37 ºC. No growth at 15
or 50 ºC. Can grow in 3%, but not in 5% NaCl. Grow at pH 6.5-9 (optimum pH 7.5),
but not at pH 5-6.
The type strain was isolated from soil in Thailand.
- Khianngam S., Akaracharanya A., Tanasupawat S., Lee J.-S. and Lee K.C., 2009. Paenibacillus thailandensis sp. nov. and
Paenibacillus nanensis sp. nov., xylanase-producing bacteria from Thai soil. IJSEM 59,564-568.
Positive results for xylanase, catalase, oxidase, hydrolysis of esculin, hydrolysis of starch, hydrolysis of Tween 80, hydrolysis of urea,
nitrate reduction, Voges-Proskauer, acid production from: N-acetylglucosamine, adonitol, aesculin, amygdalin, L- or D-arabinose,
arabitol, arbutin, cellobiose, dulcitol, erythritol, fructose, D- or L-fucose, galactose, beta-gentiobiose, gluconate, methyl-alpha-D-
glucoside, D-glucose, glycerol, glycogen, inositol, inulin, 5-ketogluconate, lactose, lyxose, methyl-alpha-D-mannoside, melezitose,
melibiose, raffinose, ribose, salicin, sorbitol, sorbose, starch, tagatose, turanose, xylitol, D- or L-xylose & methyl-beta-D-xyloside.
Negative results for arginine dihydrolase, citrate utilization, DN-ase, dihydroxyacetone production, H2S production, hydrolysis of
casein, hydrolysis of gelatin, indole production, methyl red, degradation of tyrosine, acid production from: adonitol, 2-ketogluconate,
maltose, D-mannitol, mannose, rhamnose, sucrose & trehalose.
(c) Costin Stoica