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Phylum Firmicutes, Class Bacilli, Order Bacillales, Family Bacillaceae, Genus Pullulanibacillus => Pullulanibacillus naganoensis
(Tomimura, Zeman, Frankiewicz and Teague 1990)Hatayama, Shoun, Ueda and Nakamura 2006.
Basonym: Bacillus  naganoensis Tomimura, Zeman, Frankiewicz and Teague 1990.
Description is based upon a single strain.
Gram-positive, non-motile rods, 0.5-1.0 x 2.1-10.0 µm. Occur singly or in chains. Form
subterminally, ellipsoidal endospores in swollen sporangia. Aerobic.
Show respiratory growth As (V) (arsenate) and nitrate as electron acceptors. Colonies
are  round , smooth, opaque, glistening, 2.0-3.0 mm in diameter.
Growth is observed at pH 4 - 6; temperature range 28-40 ºC. NaCl is not required for
growth, 2% NaCl tolerance. Allantoin or urate are not required.
Isolated from soil by selection using a pullulan-containing medium.
Not pathogenic to mice.
  1. N.A. Logan and P. De Vos, 2009. Genus I.  Bacillus  Cohn 1872. In: (Eds.) P.D. Vos, G. Garrity, D. Jones, N.R. Krieg, W. Ludwig, F.
    A. Rainey, K.-H. Schleifer, W.B. Whitman. Bergey’s Manual of Systematic Bacteriology, Volume 3: The Firmicutes, Springer, 21-127.
  2. E . TommuraA, N. W. Zeman, J.R.  Frankiewicz, and  W.M.  Teague, 1990. Description of Bacillus naganoensis sp. nov. IJSB 40, 2,
    123-125.
  3. 3. K. Hatayama, H. Shoun,Y. Ueda and A. Nakamura, 2006. Tuberibacillus calidus gen. nov., sp. nov., isolated from a compost pile
    and reclassification of Bacillus naganoensis  Tomimura et al. 1990 as Pullulanibacillus naganoensis gen. nov., comb.nov. and
    Bacillus laevolacticus Andersch et al.1994 as Sporolactobacillus laevolacticus comb.nov. IJSEM 56, 2545-2551.
Positive results for hydrolysis of pullulan (a thermostabile pullulanase is produced), hydrolysis of gelatin, catalase, hydrolysis of
casein, hydrolysis of starch, acid production (after 14 days incubation) from: L-arabinose, D-glucose, D-mannitol, lactose (weak) &
D-xylose.

Negative results for degradation of tyrosine, nitrate reduction, deamination of phenylalanine, hydrolysis of hippurate, egg yolk reaction,
oxidase, Voges-Proskauer & citrate utilization.
(c) Costin Stoica
Pullulanibacillus naganoensis
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