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Phylum Firmicutes, Class Bacilli, Order Bacillales, Family Bacillaceae, Genus Bacillus, Bacillus methanolicus  Arfman, Dijkhuizen,
Kirchhof, Ludwig, Schleifer, Bulygina, Chumakov, Govorhukina, Trotsenko, White & Sharp 1992.
Gram-positive rods, nonmotile. Filamentous cells may be seen in older cultures.
Produce centrally to subterminally, ellipsoidal endospores in swollen sporangia.
Colonies have rough surfaces and crenated, undulating margins on TSA. Aerobic.
Grow at pH 7-10, not at pH 6. Thermotolerant; temperature range 35-60 ºC (optimum
55 ºC). NaCl, allantoin or urate are not required for growth. Variable growth in 2%
NaCl medium, and negative in 5% NaCl.
Isolated from soil, aerobic and thermophilic wastewater treatment systems and volcanic hot springs.
Not determined.
  1. N.A. Logan and P. De Vos, 2009. Genus I. Bacillus Cohn 1872. In: (Eds.) P.D. Vos, G. Garrity, D. Jones, N.R. Krieg, W. Ludwig, F.A.
    Rainey, K.-H. Schleifer, W.B. Whitman. Bergey’s Manual of Systematic Bacteriology, Volume 3: The Firmicutes, Springer, 21-127.
  2. N. Arfman, L. Dijkhuizen, G. Kirchhof, W. Ludwig,K.-H. Schleifer, E. S. Bulygina, K.M. Khumakov, N.I. Govorukhina, Y. A. Trotsenko,
    D. White and R. J. Sharp, 1992. Bacillus methanolicus sp. nov., a New Species of Thermotolerant, Methanol-Utilizing, Endospore-
    Forming Bacteria. IJSB 42, 3, 439-445.
Positive results for catalase, oxidase, acid production from: D-glucose & D-mannitol.

Negative results for hydrolysis of hippurate, nitrate reduction, hydrolysis of casein, acid production from: glycerol, glycogen, salicin,
starch, adonitol, erythritol, D-galactose, meso-inositol, inulin, lactose, sucrose & L-xylose.

Variable results for hydrolysis of starch, acid production from: D-fructose, maltose, raffinose, D-ribose, sorbitol & trehalose.
(c) Costin Stoica
Bacillus methanolicus
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