Phylum Firmicutes, Class Bacilli, Order Bacillales, Family Bacillaceae, Genus Bacillus, Bacillus isabeliae Albuquerque, Tiago,
Taborda, Nobre, Verissimo and da Costa 2008.
Gram-positive curved rods, 0.5-1.0 x 2.8-5.7 µm, nonmotile, rarely observed
subterminally endospores in swollen sporangia.
Colonies on Degryse 162 medium containing 5% NaCl are orange. Growth factors
are required for growth. Aerobic. Grow at pH 6.5-8.5 (optimum pH 8.0). Temperature
range 20-40 ºC; no growth at 15 or 45 ºC. Halophilic, NaCl is required for growth.
Optimum growth occurs in nutrient broth with 4-6% NaCl added.
Isolated from a sea salt evaporation pond on the Island of Sal in the Cape Verde Archipelago.
- Albuquerque L., Tiago I., Taborda M., Nobre M.F., Verissimo A. and da Costa M.S. 2008. Bacillus isabeliae sp. nov., a halophilic
bacterium isolated from a sea salt evaporation pond. IJSEM 58, 226-230.
Positive results for hydrolysis of esculin, DN-ase, esterase lipase, oxidase, catalase,
hydrolysis of starch, hydrolysis of xylan, acid production from: cellobiose, fructose
(weak), beta-gentiobiose, 5-ketogluconate, glycogen, melezitose, ribose (weak),
sorbose (weak), sucrose (weak), trehalose & D-xylose (weak).
Negative results for arginine dihydrolase, beta-galactosidase, lysine decarboxylase, hydrolysis of hippurate, hydrolysis of gelatin,
nitrate reduction, hydrolysis of casein, citrate utilization, hydrolysis of Tween 20 and Tween 80, alkaline phosphatase, esterase,
lipase, trypsin, acid phosphatase, acid production from: D- or L-arabinose, N-acetyl-D-glucosamine, adonitol, amygdalin, D- or
L-arabitol, arbutin, erythritol, D- or L-fucose, glycerol, galactose, D-glucose, gluconate, meso-inositol, inulin, 2-ketogluconate, lactose,
lyxose, maltose, D-mannitol, D-mannose, melibiose, raffinose, rhamnose salicin, sorbitol, starch, xylitol & L-xylose.
(c) Costin Stoica