Psychrobacillus insolitus
Taxonomy
Morphology
Cultural characteristics
Biochemical characters
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References
Phylum Firmicutes, Class Bacilli, Order Bacillales, Family Bacillaceae, Genus Psychrobacillus => Psychrobacillus insolitus (Larkin
and Stokes 1967) Krishnamurthi et al. 2011.
Basonym:
Bacillus insolitus  Larkin and Stokes (1967).
Gram positive, motile  rods or coccoid rods, 1.0-1.5 x 1.6-2.7 μm, on nutrient agar and 0.7-0.9 x 2.4-5.3 µm on trypticase soy agar. One
polar and one subpolar flagellum. Nonmotile cocci may be found. Sporulates and germinates at 0 ºC. Spores are spherical to
cylindrical, 0.7 x 1.4 µm in diameter. Terminal ellipsoidal and cylindrical spores are formed in rod-shaped cells. Not swelling the
sporangia.
Colonies on nutrient agar are small, soft, off-white and irregular. Maximum growth
temperature 25 ºC, minimum 0-5 ºC. Grow in 2% NaCl (variable) and at pH 7. Do not
grow in anaerobic agar, in  5-10% NaCl or at pH 5.7. NaCl, allantoin or urate are not
required for growth.
Isolated from normal and marshy soil. No growth in the presence of lysozyme.
Not determined.
  1. Gordon R.E., Haynes W.C., Pang C.H. (1973) – The genus Bacillus . Agriculture Handbook No. 427, U.S.D.A., Washington D.C.
  2. Buchanan R.E., Gibbons N.E., Cowan S.T., Holt J.G., Liston J., Murray R.G.E., Niven C.F., Ravin A.W., Stanier R.W. ( 1974) – Bergey’s
    Manual of Determinative Bacteriology, Eight Edition, The Williams & Wilkins Company, Baltimore.
  3. N.A. Logan and P. De Vos, 2009. Genus I.  Bacillus  Cohn 1872. In: (Eds.) P.D. Vos, G. Garrity, D. Jones, N.R. Krieg, W. Ludwig, F.A.
    Rainey, K.-H. Schleifer, W.B. Whitman. Bergey’s Manual of Systematic Bacteriology, Volume 3: The Firmicutes, Springer, 21-127.
  4. Euzeby J.P. - "List of Bacterial Names with Standing in Nomenclature". http://www.bacterio.cict.fr/b/bacillus.html
Positive for catalase, oxidase, hydrolysis of esculin, beta-galactosidase, acid
production from: glycerol, starch, N-acetyl-D-glucosamine, amygdalin, arbutin,
cellobiose, fructose, beta-gentibiose, maltose, ribose & sucrose.

Negative results for starch hydrolysis, reduction of nitrate to nitrite, decomposition of
casein, decomposition of tyrosine, Voges-Proskauer reaction, arginine dihydrolase,
lysine decarboxylase, ornithine decarboxylase, tryptophan deaminase citrate utilization,
indole production, hydrolysis of gelatin, acid production from methyl beta-xyloside,
glucose, mannitol, D-mannose, adonitol, D- or L-arabinose, L-arabitol, dulcitol,
erythritol, D- or L-fucose, gluconate, 2- or 5-ketogluconate, salicin, sorbose, xylitol & xylose.

Variable results for deamination of phenylalanine, hydrolysis of urea, acid production from: glycogen, D-arabitol, galactose,
meso-inositol, inulin, lactose, lyxose, melezitose , melibiose, raffinose, rhamnose, sorbitol & trehalose.
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