Phylum Firmicutes, Class Bacilli, Order Bacillales, Family Bacillaceae, Genus Bacillus, Bacillus horti Yumoto, Yamazaki, Sawabe,
Nakano, Kawasaki, Ezura and Shinano 1998.
Gram-negative rods, 0.6-0.8 x 1.5-6.0 µm, motile by peritrichous flagella. Produce
subterminal, ellipsoidal endospores in swollen sporangia.
Colonies are white on complex medium at pH 10. Aerobic. Growth occurs at pH 7 to
10 (optimum pH 8-10) and at 15 - 40 ºC. Grow in nutrient broth with 2-11% NaCl
added. Allantoin and urate are not required for growth. NaCl requirement is variable.
Isolated from garden soil in Hokkaido, Japan.
- N.A. Logan and P. De Vos, 2009. Genus I. Bacillus Cohn 1872. In: (Eds.) P.D. Vos, G. Garrity, D. Jones, N.R. Krieg, W. Ludwig, F.
A. Rainey, K.-H. Schleifer, W.B. Whitman. Bergey’s Manual of Systematic Bacteriology, Volume 3: The Firmicutes, Springer, 21-127.
- Yumoto I., Yamazaki K., Sawabe T., Nakano K., Kawasaki K., Ezura Y. and Shinano H., 1998. Bacillus horti sp. nov., a new Gram-
negative alkaliphilic bacillus. IJSB 48, 565-571.
Positive results for β-galactosidase, hydrolysis of gelatin, oxidase, catalase, nitrate
reduction, hydrolysis of casein, hydrolysis of starch, hydrolysis of DNA, acid production
from: D-glucose, D-xylose, fructose & ribose.
Negative results for deamination of phenylalanine, hydrolysis of urea, indole production,
H2S production (positive at pH 7), hydrolysis of Tween 20, 40, 60 & 80, acid production from: D-arabinose, erythritol, galactose, meso-
inositol, melibiose, raffinose, rhamnose, sorbitol & sucrose.
Variable results for hydrolysis of hippurate, acid production from D-mannose, lactose
(c) Costin Stoica