Phylum Firmicutes, Class Bacilli, Order Bacillales, Family Bacillaceae, Genus Bacillus, Bacillus gelatini De Clerck, Rodriguez-Diaz,
Vanhoutte, Heyman, Logan and De Vos 2004.
Gram-variable, motile, straight rods, 0.5-0.9 x 4.0-10.0 µm, forms paracentrally or
subterminally, ellipsoidal endospores in unswollen sporangia.
Colonies are smooth, opaque , cream but darker in the center, with slightly irregular
borders; are waxy in appearance, with eggshell-textured surfaces, slightly convex
(older colonies are flatter with concave, transparent centers, and 1-4 mm diameter on
TSA at 30 ºC in 4 days. Aerobic.
Growth at pH 4 - 10 & at 30 - 60 ºC (40 - 50 ºC optimum). Allantoin, urate or NaCl are
not required for growth. Good growth occurs in nutrient broth with 15% NaCl added.
Isolated from gelatin production plants.
- N.A. Logan and P. De Vos, 2009. Genus I. Bacillus Cohn 1872. In: (Eds.) P.D. Vos, G. Garrity, D. Jones, N.R. Krieg, W. Ludwig, F.
A. Rainey, K.-H. Schleifer, W.B. Whitman. Bergey’s Manual of Systematic Bacteriology, Volume 3: The Firmicutes, Springer, 21-127.
- De Clerck E., Rodriguez-Diaz M., Vanhoutte T., Heyrman J., Logan N. A. and De Vos P. 2004. Anoxybacillus contaminans sp. nov.
and Bacillus gelatini sp. nov., isolated from contaminated gelatin batches. IJSEM 54, 941-946.
Positive results for catalase, hydrolysis of esculin, hydrolysis of gelatin, hydrolysis of
casein, acid production from: D-glucose, D-mannitol, D-mannose, glycerol, D-xylose,
N-acetyl-D-glucosamine (weak), fructose, maltose (weak), ribose (weak) & trehalose.
Negative results for oxidase, beta-galactosidase, arginine dihydrolase, lysine decarboxylase, ornithine decarboxylase, hydrolysis of
urea, indole production, H2S production, nitrate reduction, Voges-Proskauer reaction, citrate utilization, tryptophan deaminase, acid
production from: D- or L-arabinose, adonitol , amygdalin, D- or L-arabitol, arbutin, dulcitol, erythritol, D- or L-fucose, beta-gentibiose,
gluconate, glycogen, inulin, 2-ketogluconate, lactose, lyxose, methyl beta-xyloside, melibiose, raffinose, rhamnose, salicin, starch,
sorbitol, sorbose, sucrose, xylitol & L-xylose.
Variable results for acid production from: cellobiose, galactose, meso-inositol, 5-ketogluconate & melezitose.
(c) Costin Stoica