Phylum Firmicutes, Class Bacilli, Order Bacillales, Family Bacillaceae, Genus Bacillus, Bacillus flexus (ex Batchelor 1919) Priest,
Goodfellow and Todd 1989.
Gram-variable, rods, 0.9 µm mean cell width, presenting centrally / paracentrally,
ellipsoidal endospores in unswollen sporangia.
Colonies are opaque and smooth. Aerobic. No anaerobic growth. Grow at pH 4.5 -
9.5 and in 2 - 10 % NaCl. Growth temperature 17 to 37 ºC (optimum 30 ºC). Allantoin,
urate or NaCl are not required for growth.
Isolated from feces (poultry) and soil.
- N.A. Logan and P. De Vos, 2009. Genus I. Bacillus Cohn 1872. In: (Eds.) P.D. Vos, G. Garrity, D. Jones, N.R. Krieg, W. Ludwig, F.
A. Rainey, K.-H. Schleifer, W.B. Whitman. Bergey’s Manual of Systematic Bacteriology, Volume 3: The Firmicutes, Springer, 21-127.
Positive results for beta-galactosidase, hydrolysis of urea, hydrolysis of gelatin,
oxidase, hydrolysis of casein, hydrolysis of starch, citrate utilization, hydrolysis of
Tween 20, acid production from: D-glucose, D-mannitol, glycerol, fructose, galactose,
lactose, maltose, raffinose, sucrose & trehalose.
Negative results for deamination of phenylalanine, hydrolysis of esculin, hydrolysis of hippurate, egg yolk reaction, indole production,
nitrate reduction, Voges-Proskauer reaction, degradation of tyrosine, acid production from: L-arabinose, adonitol, D- cellobiose,
dulcitol, erythritol, D-mannose, rhamnose, sorbitol, D-xylose & L-xylose.
Variable results for hydrolysis of Tween 80, meso-inositol & salicin acidification.
(c) Costin Stoica