Phylum Firmicutes, Class Bacilli, Order Bacillales, Family Bacillaceae, Genus Bacillus, Bacillus drentensis Heyman, Vanparys,
Logan, Balcaen, Rodriguez-Diaz, Felske and De Vos 2004.
It was separated from B. lentus by a difference of only 5% in similarity, therefore it is
not easily distinguishable from B. lentus by phenotypic tests.
Gram-positive or Gram-variable tapered rods, 0.6-1.2 µm in diameter, motile. Cells
show pleomorphism (narrow and broad cells, the latter showing swellings) and
produce intracellular storage products on TSA. Produce paracentral or subterminal,
spherical / ellipsoidal endospores in swollen sporangia.
Colonies are cream and produce a brownish soluble pigment ; consistency is
butyrous with an eggshell-like surface texture; colonies are slightly convex with
regular margins when small and sometimes wrinkled with irregular margins and
prominent centres when larger. Facultatively anaerobic.
Grow at pH 6 - 9 (optimum pH 7 - 8), variable at 5.5 & 10. Growth temperature 30 ºC
(optimum) to 55 ºC (variable). Allantoin, urate or NaCl are not required for growth.
Isolated from soil in the Drentse, an agricultural research area, The Netherlands.
- N.A. Logan and P. De Vos, 2009. Genus I. Bacillus Cohn 1872. In: (Eds.) P.D. Vos, G. Garrity, D. Jones, N.R. Krieg, W. Ludwig, F.
A. Rainey, K.-H. Schleifer, W.B. Whitman. Bergey’s Manual of Systematic Bacteriology, Volume 3: The Firmicutes, Springer, 21-127.
- HeyrmanJ., Vanparys B., Logan N.A., Balcaen A., Rodriguez-Diaz M., Felske A. and De Vos P., 2004. Bacillus novalis sp. nov.,
Bacillus vireti sp. nov.,Bacillus soli sp. nov., Bacillus bataviensis sp. nov. and Bacillus drentensis sp. nov., from the Drentse A
grasslands. IJSEM 54, 47-57.
Positive results for beta-galactosidase, hydrolysis of esculin, acid production from: D-glucose (weak), N-acetyl-D-glucosamine,
fructose, lactose, maltose, melibiose & salicin (weak).
Negative results for arginine dihydrolase, lysine decarboxylase, ornithine decarboxylase, hydrolysis of gelatin, indole production,
H2S production, hydrolysis of casein, citrate utilization, tryptophan deaminase, acid production from: L- or D-arabinose, adonitol, L- or
D-arabitol, cellobiose, dulcitol, erythritol, glycerol, glycogen, L- or D-fucose, beta-gentibiose, meso-inositol, 2- or 5-ketogluconate,
lyxose, D-mannitol, methyl beta-xyloside, rhamnose, sorbitol, sorbose, xylitol & L-xylose.
Variable or weak results for Voges-Proskauer test, nitrate reduction, acid production from: amygdalin, arbutin, galactose, gluconate,
D-mannose, melezitose, raffinose, sucrose, trehalose & D-xylose.
(c) Costin Stoica