Phylum Firmicutes, Class Bacilli, Order Bacillales, Family Bacillaceae, Genus Bacillus, Bacillus decolorationis Heyman, Balcaen,
Rodriguez-Diaz, Logan, Swings and De Vos 2003.
Gram-variable rods and coccoid rods, 0.5-0.8 x 1.0-4.0 µm, motile. Central to
subterminal, ellipsoidal/nearly spherical endospores in slightly swollen sporangia.
Spores are produced slowly and in small number in culture.
Colonies are cream to beige, circular with a smooth to slightly irregular margin,
low-convex with a glistening and rough surface on TSA.
Aerobic. Grow at pH 6 - 7 and in 0 to 10% NaCl (optimum 4-7%). Growth temperature
5 - 40 ºC (optimum 25- 37 ºC). Allantoin, urate or NaCl are not required for growth.
Isolated from biodeteriorated parts of the mural paintings discolored by microbial growths at the Servilia tomb (Roman necropolis of
Carmona, Spain) and the Saint-Catherine chapel (Castle Herbestein, Austria).
- N.A. Logan and P. De Vos, 2009. Genus I. Bacillus Cohn 1872. In: (Eds.) P.D. Vos, G. Garrity, D. Jones, N.R. Krieg, W. Ludwig, F.
A. Rainey, K.-H. Schleifer, W.B. Whitman. Bergey’s Manual of Systematic Bacteriology, Volume 3: The Firmicutes, Springer, 21-127.
- Heyrman J., Balcaen A., Rodriguez-Diaz M., Logan N. A., Swings J. and De Vos P., 2003. Bacillus decolorationis sp. nov., isolated
from biodeteriorated parts of the mural paintings discolored by microbial growths at the Servilia tomb (Roman necropolis of
Carmona, Spain) and the Saint-Catherine chapel (Castle Herbestein, Austria). IJSEM 53, 459-463.
Positive results for hydrolysis of esculin, hydrolysis of gelatin (large inoculum),
oxidase, catalase, nitrate reduction & hydrolysis of casein (within 4 days).
Weak reaction for acid production in 7% NaCl medium from: D-glucose, D-mannose, salicin, N-acetil-D-glucosamine, cellobiose,
fructose, beta-gentibiose, 5-ketogluconate, maltose, sucrose & trehalose.
Negative results for arginine dihydrolase, beta-galactosidase (ONPG) without added NaCl ( variable results in 7%NaCl medium),
lysine decarboxylase, ornithine decarboxylase, urease, indole production, H2S production, Voges-Proskauer test, citrate utilization &
No acid production in 7% NaCl medium from: L- or D-arabinose, adonitol, amygdalin, L- or D-arabitol, dulcitol, erythritol, L- or
D-fucose, glycogen, gluconate, meso-inositol, inulin, 2-ketogluconate, lyxose, melezitose, melibiose, raffinose, rhamnose, sorbitol,
sorbose, starch, xylitol, L- or D-xylose.
Variable and weak results for acid production in 7% NaCl medium from: D-mannitol, arbutin & galactose.
(c) Costin Stoica