Phylum Firmicutes, Class Bacilli, Order Bacillales, Family Paenibacillaceae, Genus Paenibacillus, Paenibacillus contaminans Chou,
Lee, Lin, Chang, Arun, Young and Chen 2009.
Gram-variable, motile (peritrichous flagella) rods, 0.8–1.0 x 2.0–3.0 μm. Produce
ellipsoidal; paracentral/subterminal spores in swollen sporangium.
Colonies on nutrient agar are colourless, semi-transparent,approximately 0.5–1.0
mm in diameter after 72 hours incubation at 30 ºC. Grow both aerobically and
anaerobically. Grow in 0-2% NaCl but not in 5% NaCl medium. Grow at 10-37 ºC, pH
Isolated from a contaminated laboratory plate. Resistant to sulfamethoxazole plus trimethoprim. Sensitive to ampicillin,
chloramphenicol, gentamicin, kanamycin, nalidixic acid, novobiocin, penicillin G, rifampicin, streptomycin and tetracycline.
- Chou, J.-H., Lee, J.-H., Lin, M.-C., Chang, P.-S., Arun, A.B., Young, C.-C. and Chen, W.-M., 2009. Paenibacillus contaminans sp. nov.,
isolated from a contaminated laboratory plate. IJSEM 59, 125-129.
Positive results for catalase, oxidase, DNase, hydrolysis of aesculin, Voges-Proskauer
test, citrate utilization, beta-galactosidase, acid production from: L-arabinose (weak),
D-glucose (weak) & rhamnose.
Negative results for hydrolysis of urea, nitrate reduction, hydrolysis of starch, hydrolysis of casein, hydrolysis of Tween 20, 40, 60 & 80,
hydrolysis of gelatin, lysine decarboxylase, arginine dihydrolase, ornithine decarboxylase, indole production, H2S production, acid
production from: amygdalin, inositol, lactose, D-mannitol, melibiose, sorbitol, sucrose & xylose.
(c) Costin Stoica