Phylum Firmicutes, Class Bacilli, Order Bacillales, Family Bacillaceae, Genus Bacillus, Bacillus cohnii Spanka and Fritze 1993.
Gram-positive, peritrichously motile rods, 0.6-0.7 µm cell width, subterminally to
terminally, ellipsoidal endospores in swollen sporangia.
Colonies are creamy-white on alkaline meat extract-peptone medium, 1-2 mm in
diameter (2 days at 45 ºC). Obligately alkaliphilic. Grow at pH 9-10 ( 9.7 optimum).
Grows in 2 and 5% NaCl. Growth temperature 10-47 ºC. Allantoin, urate or NaCl are
not required for growth.
Isolated from soil and feces (of old horses), Denmark and Germany; one strain was contaminant on an agar plate in Great Britain.
- N.A. Logan and P. De Vos, 2009. Genus I. Bacillus Cohn 1872. In: (Eds.) P.D. Vos, G. Garrity, D. Jones, N.R. Krieg, W. Ludwig, F.A.
Rainey, K.-H. Schleifer, W.B. Whitman. Bergey’s Manual of Systematic Bacteriology, Volume 3: The Firmicutes, Springer, 21-127.
- Spanka R. and Fritze D., 1993. Bacillus cohnii sp. nov. , a New, Obligately Alkaliphilic, Oval-Spore-Forming Bacillus Species with
Ornithine and Aspartic Acid Instead of Diaminopimelic Acid in the Cell Wall. IJSB 43, 1, 150-156.
Positive results for hydrolysis of hippurate, hydrolysis of gelatin, oxidase, catalase,
nitrate reduction, hydrolysis of casein, hydrolysis of starch, hydrolysis of Tween 20, 60
& 80, beta-galactosidase.
Negative results for deamination of phenylalanine & hydrolysis of urea.
Strain didn't grow when testing Voges-Proskauer reaction & acid production from: L-arabinose, D-glucose, D-mannitol, D-mannose,
methyl beta-xyloside, glycerol, glycogen, salicin, starch and D-xylose.
(c) Costin Stoica