Phylum Firmicutes, Class Bacilli, Order Bacillales, Family Bacillaceae, Genus Bacillus, Bacillus carboniphilus Fujita, Shida, Takagi,
Kunugita, Pankrushina and Matsuhashi 1996.
Gram-positive rods, 0.5-0.9/3.0-5.0 µm, motile by means of peritrichous flagella. Form
terminally or centrally ellipsoidal endospores, not swelling the sporangia.
Colonies on nutrient agar are circular, flat, smooth and grayish yellow; brown-red
pigment is produced on tryptone-soya agar. Growth is promoted by activated carbon
and graphite. No anaerobic growth. NaCl, allantoin or urate are not required for
growth. Grow at pH 5.6-7 and 2-7% NaCl. Growth temperature is 17-47 ºC. No growth
at 10 or 50 ºC.
Isolated from air using antibiotic-containing medium spotted with sterile graphite.
- N.A. Logan and P. De Vos, 2009. Genus I. Bacillus Cohn 1872. In: (Eds.) P.D. Vos, G. Garrity, D. Jones, N.R. Krieg, W. Ludwig, F.
A. Rainey, K.-H. Schleifer, W.B. Whitman. Bergey’s Manual of Systematic Bacteriology, Volume 3: The Firmicutes, Springer, 21-127.
- T. Fujita, O. Shida, H. Takagi, K. Kunugita, A.N. Pankrushina, and M. Matsuhashi, 1996. Description of Bacillus carboniphilus sp.
nov. IJSB 46, 116-118.
Positive results for oxidase, catalase, hydrolysis of hippurate, hydrolysis of gelatin,
hydrolysis of casein, hydrolysis of starch & hydrolysis of Tween 80.
Negative results for arginine dihydrolase, beta-galactosidase, lysine decarboxylase, ornithine decarboxylase, hydrolysis of urea,
ONPG, nitrate reduction, Voges-Proskauer, citrate, acid production from: L-arabinose, D-glucose, D-mannitol, D-mannose, glycerol,
salicin, starch, D-xylose, adonitol, cellobiose, erythritol, fructose, galactose, meso-inositol, lactose, maltose, melibiose, raffinose,
rhamnose, ribose, sorbose, sucrose & trehalose.
(c) Costin Stoica