Phylum Firmicutes, Class Bacilli, Order Bacillales, Family Bacillaceae, Genus Bacillus, Bacillus benzoevorans Pichinoty,
Asselineau and Mandel 1987.
Gram-variable rods and filaments, 1.8 µm diameter, motile. Forms ellipsoidal
endospores which do not swell the sporangia.
Colonies are opaque with matt surface, circular, flat, off-white. Prototrophic, use
aromatic acids and fenols, but not carbohydrates and aminoacids (excepting glycine)
as carbon and energy sources. Grows in media containing yeast extract and
Na-acetate or benzoate. Growth is observed at pH 7; optimum temperature 32 ºC.
NaCl, allantoin or urate are not required for growth. No growth in 5, 7 or 10% NaCl.
Isolated from pasteurized soil by aerobic enrichment in minimal medium containing benzoate, p-hydroxybenzoate or cyclohexane
carboxylate. No growth in the presence of lysozyme.
- N.A. Logan and P. De Vos, 2009. Genus I. Bacillus Cohn 1872. In: (Eds.) P.D. Vos, G. Garrity, D. Jones, N.R. Krieg, W. Ludwig, F.
A. Rainey, K.-H. Schleifer, W.B. Whitman. Bergey’s Manual of Systematic Bacteriology, Volume 3: The Firmicutes, Springer, 21-127.
Positive results for hydrolysis of urea, hydrolysis of hippurate & nitrate reduction
(no nitrite reduction).
Negative results for degradation of tyrosine, hydrolysis of starch, Voges-Proskauer test, deamination of phenylalanine,
beta-galactosidase, hydrolysis of gelatin, oxidase, acid production from: L-arabinose, D-glucose, D-mannitol, D-mannose, methyl
beta-xyloside, glycogen, salicin, starch & D-xylose.
Variable results for catalase.
(c) Costin Stoica