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Phylum Firmicutes, Class Bacilli, Order Bacillales, Family Bacillaceae, Genus Bacillus, Bacillus barbaricus  Taubel, Kampfer,
Buczolits, Lubitz and Busse 2003.
Gram-positive  rods, 0.5 x 4-5 µm, nonmotile. Borne subterminally,  ellipsoidal
endospores  in swollen sporangia.
Colonies are brownish, circular, opaque, flat, 3-7 mm. in diameter on peptone - yeast
extract - succinate (PYES) agar. Alkalitolerant. Facultatively anaerobic.
Growth is observed at pH 6 - 9.5; temperature range 18-42 ºC (optimum 30-37 ºC).
NaCl is not required for growth, 2% NaCl tolerance. Allantoin or urate are not required.
Isolated from  experimental wall painting exposed in the Virgilkapella, Vienna, Austria.  No growth in the presence of lysozyme.
Unknown.
  1. N.A. Logan and P. De Vos, 2009. Genus I.  Bacillus  Cohn 1872. In: (Eds.) P.D. Vos, G. Garrity, D. Jones, N.R. Krieg, W. Ludwig, F.
    A. Rainey, K.-H. Schleifer, W.B. Whitman. Bergey’s Manual of Systematic Bacteriology, Volume 3: The Firmicutes, Springer, 21-127.
  2. M. Taubel, P. Kampfer, S. Buczolits, W. Lubitz, H.Jurgen Busse, 2003.Bacillus barbaricus sp. nov., isolated from an experimental
    wall paiting.IJSEM 53, 725-730.
Positive results for hydrolysis of hippurate, catalase, acid production from: D-glucose,
glycogen, starch, N-acetil-D-glucosamine,  5-ketogluconate, maltose, ribose &
trehalose.

Negative results for egg yolk reaction, oxidase, degradation of tyrosine, beta-galactosidase, hydrolysis of esculin, hydrolysis of urea,
indole, H
2S, nitrate reduction, citrate utilization hydrolysis of Tween 20, hydrolysis of Tween 80, acid production from: D-mannitol,      
methyl beta-xyloside, glycerol, salicin, adonitol, amygdalin, D-arabinose, D- or L-arabitol, arbutin, cellobiose, dulcitol, erythritol, D- or
L-fucose, beta-gentibiose, gluconate, meso-inositol, inulin, 2-ketogluconate, lyxose, melezitose, melibiose, raffinose, rhamnose,
sorbitol, sorbose, D- or L-xylose & xylitol.

Variable results for acidification of fructose, galactose, lactose & sucrose.
(c) Costin Stoica
Bacillus barbaricus
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