Phylum Firmicutes, Class Bacilli, Order Bacillales, Family Bacillaceae, Genus Bacillus, Bacillus azotoformans Pichinoty, de Barjac,
Mandel and Asselineau 1983.
Gram-negative or positive rods, 0.5-0.8 x 3.0-7.0 µm, motile by means of peritrichous
flagella. Terminal or subterminal ellipsoidal endospores (1.4 x 2-2.5 µm), swelling
Colonies are partially translucent, with entire margins, up to 5 mm in diameter on
yeast extract agar. Growth requirements are complex; growth factors are required.
Aerobic. Growth is observed at pH 6 - 7; temperature range 30 to 42-46 ºC. NaCl is
not required for growth; no growth in 5-7% NaCl. Allantoin or urate are not required.
Isolated from garden soil by enrichment culture in peptone broth in N2O atmosphere. No growth in the presence of 0.001% lysozyme.
- G. A. Rainey, K.-H. Schleifer, W.B. Whitman. Bergey’s Manual of Systematic Bacteriology, Volume 3: The Firmicutes, Springer, 21-
- Pichinoty F., de Barjac H., Mandel M. and Asselineau J., 1983. Description of Bacillus azotoformans sp. nov. IJSB 33, 3, 660-662.
Positive results for oxidase, nitrate reduction & citrate utilization.
Negative results for arginine dihydrolase, catalase, hydrolysis of Tween 80, degradation of tyrosine, deamination of phenylalanine,
beta-galactosidase, hydrolysis of esculin, hydrolysis of urea, hydrolysis of hippurate, hydrolysis of gelatin, egg yolk reaction, hydrolysis
of starch, Voges-Proskauer test, acid production from: D-glucose, D-mannose, fructose, galactose, lactose, maltose, ribose, sorbose
(c) Costin Stoica