Phylum Firmicutes, Class Bacilli, Order Bacillales, Family Bacillaceae, Genus Bacillus, Bacillus acidiceler Peak, Duncan, Veguilla,
Luna, King, Heller, Heberlein-Larson, Reeves, Cannons, Amuso and Cattani 2007.
Gram-positive to Gram-variable rods, 0.8-1.2 x 2.0-15.0 µm, grouped in chains.
Nonmotile. Produce ellipsoidal, subterminal endospores in unswollen to overtly
swollen sporangia. A thin capsule is produced.
Colonies are 3 mm in diameter on TSA with 5% sheep blood at 30 ºC,
non-haemolytic, creamy-gray, like sintered glass in opacity, smooth, shiny,
becoming dull with a fine crepe texture in older culture, round, entire, raised to low
convex and becoming crater form ; colony diameter on pH 5.6 agar after 3 days is
larger than 5 mm. Grow on nutrient agar and nutrient broth. Aerobic. Grow at 30 ºC
and at pH 5.6-8.
No growth in 5% NaCl broth.
Isolated in Tampa, Florida, USA, from a white powder collected on a window sill and suspected of harbouring Bacillus anthracis
during the aftermath of the anthrax bioterrorism attacks of October 2001.
Resistant to penicillin, oxacillin, ampicillin, amoxicillin and ceftriaxone.
A thin capsule is produced; genes (acpA, capA, capB, capC, capR, capD, IS1627, ORF 48, ORF 61 and repA) and capsule promoter
with more than 99.7% sequence similarity to the capsule operon of Bacillus anthracis are carried on a large plasmid.
- K.K. Peak, K.E. Duncan, W. Veguilla, V.A. Luna, D.S. King, L. Heller, L. Heberlein-Larson, F. Reeves, A.C. Cannons, P. Amuso and
J. Cattani, 2007. Bacillus acidiceler sp. nov., isolated from a forensic specimen, containing Bacillus anthracis pX02 genes. IJSEM
Positive results for hydrolysis of esculin, hydrolysis of gelatin, oxidase, catalase, nitrate reduction, hydrolysis of casein, Voges -
Proskauer reaction (weak), acid production from: D-glucose, D-mannitol, D-mannose, glycerol (weak), glycogen (weak), salicin,
starch (weak), N-acetyl-D- glucosamine, amygdalin, arbutin, cellobiose, fructose, beta-gentiobiose, gluconate, maltose, sucrose &
Negative results for arginine dihydrolase, beta-galactosidase, lysine decarboxylase, ornithine decarboxylase, hydrolysis of urea,
hydrolysis of starch, indole production, citrate utilization, acid production from: D- or L-arabinose, methyl beta-xyloside, adonitol, D- or
L-arabitol, dulcitol, erythritol, D- or L-fucose, galactose, meso-inositol, inulin, 2- or 5-ketogluconate, lactose, lyxose, melezitose,
melibiose, raffinose, rhamnose, ribose, sorbitol, sorbose, xylitol, D- or L-xylose.
(c) Costin Stoica