Phylum Proteobacteria, Class Betaproteobacteria, Order Burkholderiales, Family Alcaligenaceae, Genus Pusillimonas, Pusillimonas
soli Lee et al. 2010.
Gram-negative rods, 0.3-0.5 x 0.7-1.0 μm. Motile. Non-spore-forming.
On LB agar, colonies reach 1-3 mm within 5 days at 25 or 30 ºC are yellow, irregularly
circular and low-convex. Colonies grown on R2A, TSA, MacConkey and NB agar plates
at the same temperatures reach only 0.3-0.5 mm in diameter. With the exception of
MacConkey agar, weak growth occurred on the above-mentioned complex media at 42
ºC. The optimum temperature for growth is 30 ºC. Grows at pH 5.0-9.0, optimum pH 6.5-
7.0. Can grow in 0-5% (w/v) NaCl.
Isolated from a farm soil near Daejeon, South Korea.
- Lee (M.), Woo (S.G.), Chae (M.) and Ten (L.N.): Pusillimonas soli sp. nov., isolated from farm soil. Int. J. Syst. Evol. Microbiol.,
2010, 60, 2326-2330.
- Felfoldi, T. S., Vengring, A., Keki, Z., Marialigeti, K. R., Schumann, P. and Toth, E. M. 2014. Eoetvoesia caeni gen. nov., sp. nov.,
isolated from an activated sludge system treating coke plant effluent. Int. J. Syst. Evol. Microbiol., 64, 1920-1925.
Positive results for acid and alkaline phosphatase, catalase, esterase (C4), esterase lipase (C8), leucine arylamidase, nitrate
reduction to nitrogen, naphthol-AS-BI-phosphohydrolase & oxidase.
Acid is produced from: L-arabinose, arbutin, gluconate, D-glucose, glycerol, D-ribose & beta-D-xylopyranoside.
Can utilize: acetate, DL-3-hydroxybutyrate, DL-lactate, adipate, L-alanine, citrate, L-histidine, 3-hydroxybenzoate, 4-hydroxybenzoate, myo-
inositol, itaconate, L-malate, malonate, phenylacetate, L-proline, propionate, L-rhamnose, D-ribose, L-serine, D-sorbitol, suberate &
Negative results for arginine dihydrolase, casein hydrolysis, chitin hydrolysis, alpha-chymotrypsin, cystine arylamidase, DNase, esculin
hydrolysis, HE-cellulose hydrolysis, N-acetyl-beta-glucosaminidase, alpha-fucosidase, gelatin hydrolysis, alpha- and beta-
galactosidase, alpha- and beta-glucosidase, beta-glucuronidase, indole production, lipase (C14), alpha-mannosidase, starch
hydrolysis, trypsin, urease, valine arylamidase & xylan hydrolysis.
Acid is not produced from: D- or L-fucose, maltose, trehalose, D- or L-xylose, lactose, fructose, sucrose,
No assimilation of: N-acetyl-D-glucosamine, L-arabinose, gluconate, D-glucose, glycogen, L-fucose, 2- or 5-ketogluconate, maltose,
D-mannitol, D-mannose, melibiose, salicin, sucrose, trehalose, D- and L-xylose, caprate, erythritol & glycerol.
(c) Costin Stoica