Phylum Proteobacteria, Class Betaproteobacteria, Order Burkholderiales, Family Alcaligenaceae, Genus Pusillimonas, Pusillimonas
harenae Park et al. 2011.
Gram-negative ovoid rods, 0.5-0.7 x 0.6-0.9 μm. Motile by two lateral flagella on one
Colonies on R2A agar are ivory, convex and round with entire margins. Growth occurs
at 15-45 ºC (optimum 30 ºC), at pH 5.0-9.0 (optimum 6.0-7.5) and in the presence of
0-6% (w/v) NaCl (optimum 0-3%). Strictly aerobic. Can grow on R2A, LB agar, TSA
and Marine Agar.
Isolated from beach sand from the Taean coast in South Korea.
- Park (M.S.), Park (Y.J.), Jung (J.Y.), Lee (S.H.), Park (W.), Lee (K.) and Jeon (C.O.): Pusillimonas harenae sp. nov., isolated from a
sandy beach, and emended description of the genus Pusillimonas. Int. J. Syst. Evol. Microbiol., 2011, 61, 2901-2906.
Positive results for acid phosphatase, alkaline phosphatase (weak), catalase, cystinearylamidase, esterase (C4), esterase lipase (C8),
leucine arylamidase, oxidase, trypsin (weak), alpha-chymotrypsin (weak), naphthol-AS-BI-phosphohydrolase (weak), urease & valine
Can assimilate: malic acid & adipic acid.
Negative results for arginine dihydrolase, esculin hydrolysis, alpha-fucosidase, alpha- and beta-galactosidase, beta-glucuronidase,
alpha- and beta-glucosidase, N-acetyl-beta-glucosaminidase, gelatin hydrolysis, indole production, lipase (C14), alpha-mannosidase,
nitrate reduction & glucose acidification.
No assimilation of: D-glucose, L-arabinose, D-mannose, maltose, D-mannitol, potassium gluconate, N-acetylglucosamine, capric
acid, trisodium citrate and phenylacetic acid.
(c) Costin Stoica