Phylum Proteobacteria, Class Betaproteobacteria, Order Burkholderiales, Family Alcaligenaceae, Genus Pigmentiphaga,
Pigmentiphaga daeguensis Yoon et al. 2007.
Gram-negative rods, 0.3-0.6 x 0.7-2.5 μm. Non-motile. Non-spore-forming.
Colonies on TSA are circular to slightly irregular, yellowish-white in colour and 1.0-2.0
mm in diameter after 3 days incubation at 37 ºC. Growth occurs at 15 and 46 ºC, but
not at 10 or 47 ºC. Growth occurs at pH 5.0 and 9.0, optimally at pH 7-8, but nnot at pH
44.5 or 9.5. Growth occurs in the presence of 0-5% (w/v) NaCl; optimally in the
presence of 0.5% (w/v) NaCl. No anaerobic growth occurs on TSA or on TSA
supplemented with nitrate.
Isolated from wastewater from a dye works at Daegu, Korea. Susceptible to tetracycline, chloramphenicol, gentamicin, kanamycin,
neomycin and polymyxin. Resistant to carbenicillin, ampicillin, cephalothin, lincomycin, novobiocin, oleandomycin, penicillin G and
- Yoon J.H., Kang S.J., Kim W. and Oh T.K.: Pigmentiphaga daeguensis sp. nov., isolated from wastewater of a dye works, and
emended description of the genus Pigmentiphaga. Int. J. Syst. Evol. Microbiol., 2007, 57, 1188-1191.
Positive results for arginine dihydrolase (weak), catalase, esterase (C4), esterase lipase (C8), leucine arylamidase,
alpha-chymotrypsin, naphthol-AS-BI-phosphohydrolase (weak) & oxidase.
Can utilize: adipate, citrate & malate.
Negative results for acid and alkaline phosphatase, lipase (C14), valine arylamidase, cystine arylamidase, trypsin, alpha- and
beta-galactosidase, beta-glucuronidase, alpha- and beta-glucosidase, N-acetyl-beta-glucosaminidase, alpha-mannosidase,
alpha-fucosidase, lysine decarboxylase, ornithine decarboxylase, tryptophan deaminase, nitrate reduction, esculin hydrolysis, casein
hydrolysis, gelatin hydrolysis, starch hydrolysis, Tweens 20, 40, 60 and 80 hydrolysis, tyrosine hydrolysis, urea hydrolysis & xanthine
No utilization of: D- and L-arabinose, ribose, D- and L-xylose, adonitol, galactose, glucose, fructose, mannose, rhamnose, inositol,
mannitol, sorbitol, N-acetylglucosamine, arbutin, salicin, cellobiose, maltose, melibiose, sucrose, trehalose, glycerol, erythritol,
methyl beta-D-xyloside, sorbose, dulcitol, methyl alpha-D-mannoside, methyl alpha-D-glucoside, amygdalin, esculin, lactose, inulin,
melezitose, raffinose, starch, glycogen, xylitol, gentiobiose, D-turanose, D-lyxose, D-tagatose, D- and L-fucose, D- and L-arabitol,
gluconate, 2- and 5-ketogluconate, caprate & phenylacetate.
(c) Costin Stoica