Phylum Proteobacteria, Class Betaproteobacteria, Order Burkholderiales, Family Alcaligenaceae, Genus Pigmentiphaga,
Pigmentiphaga aceris Lee 2017.
Gram-negative rods, 0.8-0.9 x 1.6-2.3 μm. Motile by means of lophotrichous flagella.
Colonies are cream-coloured, circular, convex with entire margins and 1-2 mm in
diameter after incubation on NA for 5 days at 30 ºC. No diffusible pigments are
produced. Good growth is observed on nutrient agar and ISP 2 agar, with poor growth
on TSA and R2A agar. Growth occurs at 10-30 ºC (optimum, 30 ºC), at pH 7-8 and in
presence of up to 2% (w/v) NaCl (optimum, 1%) in nutrient broth. Strictly aerobic.
Isolated from sap drawn from the Acer pictum Thunb. at Mount Halla in Jeju, Republic of Korea.
- Lee, S. D. 2017. Pigmentiphaga aceris sp. nov., isolated from tree sap. Int. J. Syst. Evol. Microbiol., 67, 3198-3202.
Positive results for acid and alkaline phosphatase, catalase, esterase (C4), esterase lipase (C8), leucine arylamidase, oxidase,
alpha-chymotrypsin, naphthol-AS-BI-phosphohydrolase & valine arylamidase.
Can assimilate: citrate & malate.
Negative results for arginine dihydrolase, cystine arylamidase, esculin hydrolysis, alpha-fucosidase, gelatin hydrolysis, alpha- and
beta-galactosidase, beta-glucuronidase, alpha- and beta-glucosidase, N-acetyl-beta-glucosaminidase, indole production, lipase
(C14), alpha-mannosidase, nitrate reduction, trypsin & urease.
Acid is not produced from glucose and various substrates.
No assimilation of: D-glucose, D-arabinose, D-mannose, D-mannitol, N-acetylglucosamine, maltose, gluconate, caprate, adipate &
(c) Costin Stoica