Phylum Proteobacteria, Class Betaproteobacteria, Order Burkholderiales, Family Alcaligenaceae, Genus Parapusillimonas,
Parapusillimonas granuli Kim et al. 2010, type species of the genus.
Gram-negative short rods, 1.2-2.1x 0.3-0.9 μm. Motile by means of three flagella. Non-
Colonies grown on R2A agar at 30 ºC for 5 days, are beige-coloured and circular and
have a diameter of about 2 mm. Grows on R2A agar at 25-37 ºC (optimum 30 ºC) and
at pH 6.5–7.5 (optimum 7.0). Can grow at 4 and 45 ºC. Facultatively anaerobic.
Isolated from brownish-black granules from an upflow anaerobic sludge blanket reactor used to treat brewery wastewater, Daejeon,
Republic of Korea.
- Kim (Y.J.), Kim (M.K.), Im (W.T.), Srinivasan (S.) and Yang (D.C.): Parapusillimonas granuli gen. nov., sp. nov., isolated from
granules from a wastewater-treatment bioreactor. Int. J. Syst. Evol. Microbiol., 2010, 60, 1401-1406.
- Felfoldi, T. S., Vengring, A., Keki, Z., Marialigeti, K. R., Schumann, P. and Toth, E. M. 2014. Eoetvoesia caeni gen. nov., sp. nov.,
isolated from an activated sludge system treating coke plant effluent. Int. J. Syst. Evol. Microbiol., 64, 1920-1925.
Positive results for arginine dihydrolase, catalase, esterase (C4), esterase (C8),
leucine arylamidase, oxidase, urease & valine arylamidase (weak reaction).
Can assimilate: glucose, D-sorbitol, adipate, phenylacetate, sodium acetate, lactic acid, 3-hydroxybenzoic acid, 4-hydroxybenzoic acid,
3-hydroxybutyric acid, potassium 2-ketogluconate (negative in Felfoldi's study), malate, citrate, propionic acid, valeric acid, L-proline,
L-alanine, L-serine, L-histidine itaconic acid (weak) & gluconate (negative in Felfoldi's study). Acid is produce from glucose (negative in
Negative results for acid and alkaline phosphatase, cystine arylamidase, alpha-fucosidase, alpha- and beta-glucosidase, alpha- and
beta-galactosidase, beta-glucuronidase, N-acetyl-beta-glucosaminidase, H2S production, indole production, lipase (C14),
alpha-mannosidase, methyl red test, nitrates reduction to nitrites, protease, naphthol-AS-BI-phosphohydrolase, starch hydrolysis,
trypsin, alpha-chymotrypsin & Voges-Proskauer test.
No assimilation of: glycerol, erythritol, D- and L-xylose, D-mannose, N-acetylglucosamine, trehalose,D- and L-arabitol, D- and
L-arabinose, D-and L-fucose, caprate, L-fucose, melibiose, maltose, D-mannitol, sucrose, inositol, D-ribose, L-rhamnose, salicin,
glycogen, potassium 5-ketogluconate, sodium malonate, suberic acid & gelatin.
(c) Costin Stoica