Phylum Proteobacteria, Class Betaproteobacteria, Order Burkholderiales, Family Alcaligenaceae, Genus Caenimicrobium,
Caenimicrobium hargitense Felfoldi et al. 2017, type species of the genus.
Species description is based on a single isolate.
Gram-negative, short rods, 0.6-0.8 x 1.1-2.2 µm. Non-motile.
Colonies on nutrient medium are beige-coloured, circular and raised with a diameter
of 2-3 mm. Growth occurs at pH 7-11 and 0-8% NaCl concentration. Temperature
range for growth 4-65 ºC. Aerobic.
Isolated from activated sludge from Harghita, Romania.
- Felfoldi, T., Schumann, P., Mentes, A., Keki, Z., Mathe, I. and Toth, E. M. 2017. Caenimicrobium hargitense gen. nov., sp. nov., a
new member of the family Alcaligenaceae (Betaproteobacteria) isolated from activated sludge. Int. J. Syst. Evol. Microbiol., 67, 627-
Positive results for alkaline phosphatase (weakly), catalase, esterase lipase (C8) (weakly) esterase (C4), leucine arylamidase,
naphthol-AS-BI-phosphohydrolase, nitrate reduction & oxidase.
Negative results for acid phosphatase, cystine arylamidase, alpha-fucosidase, lipase (C14), alpha- and beta-galactosidase,
beta-glucuronidase, alpha- and beta-glucosidase, N-acetyl-beta-glucosaminidase, alpha-mannosidase, starch hydrolysis, trypsin,
alpha-chymotrypsin, urease, valine arylamidase, acid production from D-glucose.
No assimilation of: erythritol, D- and L-arabinose, D-ribose, D- and L-xylose, D-adonitol, methyl beta-D-xylopyranoside, D-galactose,
D-glucose, D-fructose, D-mannose, L-sorbose, L-rhamnose, dulcitol, inositol, D-mannitol, D-sorbitol, methyl
alpha-D-mannopyranoside, methyl alpha-D-glucopyranoside, N-acetylglucosamine, salicin, cellobiose, lactose, melibiose, sucrose,
trehalose, inulin, melezitose, raffinose, starch, glycogen, xylitol, gentiobiose, turanose, D-lyxose, D-tagatose, D- and L-fucose, D- and
L-arabitol, gluconate, 2- and 5-ketogluconate, glycerol, amygdalin, arbutin, esculin (none of the carbon sources in the API 50 CH test
(c) Costin Stoica