Phylum Proteobacteria, Class Betaproteobacteria, Order Burkholderiales, Family Alcaligenaceae, Genus Brackiella, Brackiella
oedipodis Willems et al. 2002, type species of the genus.
Species description is based on a single isolate.
Gram-negative coccoid rods, 0.9-1.1 x 1.8 µm . Non-motile. Cell is surrounded by
densely studded fimbriae 280 nm in length, but flagella are not produced.
After 24 h incubation, two types of colonies were observed: a small type (colony
diameter up to 0.5 mm) and a large type (colony diameter up to 1 mm). After 2 days
growth, colonies of both types attained diameters of 1-2 mm. Colonies are
greyish-white, slightly crumbled and brittling. Grows at temperatures in the range
30-37 ºC, but not at 4, 20 or 42 ºC.
Grows on tryptic soy agar with 5% sheep blood, under aerobic conditions. Aerobic to
microaerophilic, as shown by improved growth in the atmosphere of a candle jar. No
anaerobic growth. No haemolysis produced. No growth on MacConkey agar, King B
agar, cetrimide agar, acetamide agar, milk agar or in 6.5% NaCl broth.
Isolated from the heart of a cotton-topped tamarin that had died suddenly during a tooth extraction.
Susceptible to: amikacin, ampicillin, cefotaxime, cefotiam, cefoxitin, ceftazidime, chloramphenicol, ciprofloxacin, gentamicin, kanamycin,
mezlocillin, mezlocillin+sulbactam, nalidixic acid, tetracycline, streptomycin, sulfamerazine and trimethoprim+sulfamerazine.
Isolated from the heart of a cotton-topped tamarin with endocarditis.
- Willems (A.), Gilhaus (H.), Beer (W.), Mietke (H.), Gelderblom (H.R.), Burghardt (B.), Voigt (W.) And Reissbrodt (R.): Brackiella
oedipodis gen. nov., sp. nov., Gram-negative, oxidase-positive rods that cause endocarditis of cotton-topped tamarin (Saguinus
oedipus). Int. J. Syst. Evol. Microbiol., 2002, 52, 179-186.
Positive results for alanine aminopeptidase, catalase, nitrate reduction to nitrite, methyl red, ornithine decarboxylase, oxidase, starch
hydrolysis, production of acid from: mannose & maltose.
Can utilize: Tween 40, Tween 80, methyl pyruvate, cis-aconitic acid, formic acid, alpha-hydroxybutyric acid, beta-hydroxybutyric acid,
gamma-hydroxybutyric acid, alpha-ketobutyric acid, alpha-ketoglutaric acid, alpha-ketovaleric acid, DL-lactic acid, sebacic acid,
succinic acid, bromosuccinic acid, succinamic acid, D-alanine, L-glutamic acid, glycyl-L-glutamic acid & L-serine.
Negative results for arginine dihydrolase, Simmon’s citrate, H2S (Kligler) production, indole production, growth on KCN, esculin
hydrolysis, DNase, lipase, gelatinase, beta-galactosidase, beta-glucuronidase, lysine decarboxylase, phenylalanine deaminase,
pyrase, Tween 80 hydrolysis, urease, Voges–Proskauer test, acid production from: glucose, methyl alpha-D-glucoside, adonitol,
cellobiose, D-sorbitol, dulcitol, D-xylose, erythritol, glycerol, lactose, L-arabinose, L-rhamnose, mannitol, melibiose, mucate, myo-
inositol, raffinose, saccharose, salicin & trehalose.
No utilization of acetate, malonate & tartrate (Jordan).
(c) Costin Stoica