Phylum Proteobacteria, Class Betaproteobacteria, Order Burkholderiales, Family Alcaligenaceae, Genus Bordetella, Bordetella
muralis Tazato et al. 2015.
Gram-negative coccobacilli, 0.6-0.7 x 0.8-1.0 μm. Non-spore-forming. Non-motile.
Colonies are smooth, convex, entire, opaque, glistening and pale yellow, 2–3 mm in
diameter on nutrient agar after 48 h of incubation at 30 ºC. No growth factor is required.
Can grow at pH 5.0-10.0 (optimum 7.0-8.0). Strictly aerobic. Growth temperature 10-40
ºC (optimum 25-30 ºC). Can grow on MacConkey agar and Simmons' citrate agar.
Water-soluble brown pigment on heart infusion agar containing tyrosine is not
Isolated from a cotton-swab sample collected from the surface of mural paintings on the west wall of the four ‘Asuka beauties’ (Asuka
Bijin) inside the stone chamber of Takamatsuzuka tumulus in Asuka village, Nara Prefecture, Japan.
- Tazato, N., Handa, Y., Nishijima, M., Kigawa, R., Sano, C. and Sugiyama, J. 2015. Novel environmental species isolated from the
plaster wall surface of mural paintings in the Takamatsuzuka tumulus: Bordetella muralis sp. nov., Bordetella tumulicola sp. nov.
and Bordetella tumbae sp. nov. Int. J. Syst. Evol. Microbiol., 65, 4830-4838.
Description is based based mostly on API 20NE and API ZYM (bioMerieux) results.
Positive results for acid phosphatase, catalase, esterase (C4), leucine arylamidase, nitrate reduction to nitrite, oxidase &
Can utilize: L-arabinose, D-xylose, adipate, DL-malate, citrate & phenylacetate.
Negative results for alkaline phosphatase, arginine dihydrolase, esculin hydrolysis, esterase lipase (C8), lipase (C14), gelatin
hydrolysis, alpha- and beta-galactosidase, beta-glucuronidase, alpha- and beta-glucosidase, N-acetyl-beta-glucosaminidase,
alpha-mannosidase, alpha-fucosidase, indole production, urease, valine arylamidase, cystine arylamidase, trypsin,
alpha-chymotrypsin, acid production from glucose.
No utilization of: D-glucose, D-mannose, lactose, sucrose, maltose, N-acetyl-D-glucosamine, D-mannitol, D-gluconate and n-caprate.
(c) Costin Stoica