Phylum Proteobacteria, Class Betaproteobacteria, Order Burkholderiales, Family Alcaligenaceae, Genus Ampullimonas,
Ampullimonas aquatilis Franca et al. 2016, type species of the genus.
Gram-negative rods, 1.0 x 1.0-2.0 μm. Motile. Non-spore-forming.
Colonies on R2A agar medium are not pigmented. Growth occurs between 15 and 35
ºC, optimally at 30 ºC. The optimum pH for growth is about 7.0; growth occurs
between pH 5.5 and 9.0. Optimum growth occurs without added NaCl; the
maximum concentration for growth is 0.5 % (w/v) NaCl. Strictly aerobic. Yeast extract
is required for growth.
Able to fix N2 under microaerobic conditions.
Isolated from bottled mineral water in Portugal.
- Franca, L., Albuquerque, L., Sanchez, C., Fareleira, P. and Da Costa, M. S. 2016. Ampullimonas aquatilis gen. nov., sp. nov.
isolated from bottled mineral water. Int. J. Syst. Evol. Microbiol., 66, 1459-1465.
Positive results for leucine arylamidase, nitrate reduction, oxidase, Tweens 60 & 80 hydrolysis.
Can assimilate: DL-Lactate, pyruvate, succinate & malate.
Negative results for acid and alkaline phosphatase, catalase, casein hydrolysis, esculin hydrolysis, esterase (C4), esterase lipase,
valine arylamidase, naphthol-AS-BI-phosphohydrolase, alpha- and beta-galactosidase, beta-glucuronidase, alpha- and
beta-glucosidase, alpha-mannosidase, alpha-fucosidase, N-acetyl-beta-glucosaminidase, DNase, gelatin hydrolysis, starch
hydrolysis, Tweens 20 and 40 hydrolysis, trypsin & alpha-chymotrypsin.
No assimilation of: D-glucose, D-fructose, sucrose, D-gluconate, L-glutamate, D-mannitol, D-sorbitol, cis-aconitic acid, citric acid,
formic acid, D-gluconic acid, malonic acid, propionic acid, quinic acid, D-saccharic acid, succinic acid, succinamic acid, L-glutamic
acid, L-proline, 2-aminoethanol, D-mannose, L-rhamnose, L-fucose, L-arabinose, maltose, cellobiose, raffinose, D-glucosamine,
N-acetylglucosamine, ribitol, xylitol, myo-inositol, erythritol, D-arabitol, citrate, aspartate, L-alanine, L-aspargine, L-histidine,
L-phenylalanine, L-leucine, L-ornithine, dextrin, D-galactose, trehalose, melibiose, lactose, maltose, sorbitol, alpha-ketoglutarate,
formate, L-proline & L-serine.
Variable results for the assimilation of glycerol.
(c) Costin Stoica