Taxonomy
Morphology
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Phylum Proteobacteria, Class Betaproteobacteria, Order Burkholderiales, Family Alcaligenaceae, Genus Ampullimonas,
Ampullimonas aquatilis Franca et al. 2016, type species of the genus.
Gram-negative rods, 1.0 x 1.0-2.0 μm. Motile. Non-spore-forming.
Colonies on R2A agar medium are not pigmented. Growth occurs between 15 and 35
ºC, optimally at 30 ºC. The optimum pH for growth is about 7.0; growth occurs
between pH 5.5 and 9.0. Optimum growth occurs without added NaCl; the
maximum concentration for growth is 0.5 % (w/v) NaCl. Strictly aerobic. Yeast extract
is required for growth.
Able to fix N
2 under microaerobic conditions.
Isolated from bottled mineral water in Portugal.
Undetermined.
  1. Franca, L., Albuquerque, L., Sanchez, C., Fareleira, P. and Da Costa, M. S. 2016. Ampullimonas aquatilis gen. nov., sp. nov.
    isolated from bottled mineral water. Int. J. Syst. Evol. Microbiol., 66, 1459-1465.
Positive results for leucine arylamidase, nitrate reduction, oxidase, Tweens 60 & 80 hydrolysis.
Can assimilate: DL-Lactate, pyruvate, succinate & malate.

Negative results for acid and alkaline phosphatase, catalase, casein hydrolysis, esculin hydrolysis, esterase (C4), esterase lipase,
valine arylamidase, naphthol-AS-BI-phosphohydrolase, alpha- and beta-galactosidase, beta-glucuronidase, alpha- and
beta-glucosidase, alpha-mannosidase, alpha-fucosidase, N-acetyl-beta-glucosaminidase, DNase, gelatin hydrolysis, starch
hydrolysis, Tweens 20 and 40 hydrolysis, trypsin & alpha-chymotrypsin.
No assimilation of: D-glucose, D-fructose, sucrose, D-gluconate, L-glutamate, D-mannitol, D-sorbitol, cis-aconitic acid, citric acid,
formic acid, D-gluconic acid, malonic acid, propionic acid, quinic acid, D-saccharic acid, succinic acid, succinamic acid, L-glutamic
acid, L-proline, 2-aminoethanol, D-mannose, L-rhamnose, L-fucose, L-arabinose, maltose, cellobiose, raffinose, D-glucosamine,
N-acetylglucosamine, ribitol, xylitol, myo-inositol, erythritol, D-arabitol, citrate, aspartate, L-alanine, L-aspargine, L-histidine,
L-phenylalanine, L-leucine, L-ornithine, dextrin, D-galactose, trehalose, melibiose, lactose, maltose, sorbitol, alpha-ketoglutarate,
formate, L-proline & L-serine.

Variable results for the assimilation of glycerol.
(c) Costin Stoica
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Ampullimonas aquatilis