Advenella mimigardefordensis
Cultural characteristics
Biochemical characters
Phylum Proteobacteria, Class Betaproteobacteria, Order Burkholderiales, Family Alcaligenaceae, Genus Advenella, Advenella
(Wübbeler et al. 2006) Gibello et al. 2009.

Old synonym:
Tetrathiobacter mimigardefordensis Wübbeler et al. 2006.
Gram-negative, coccoid rods, 1.5-2 μm in length. Non-spore-forming. Motile.
aggregates in chains
Colonies on 3,3'-dithiodipropionic acid (DTDP) - containing agar plates are more flat
than convex, up to 3 mm in diameter and white to lime-yellowish in colour, showing
an increase in yellow colour with culture age. On complex medium, colonies grow in
1-2 days and appear more white than yellow and are up to 5 mm in diameter.
Temperature range for growth is 15–40 ºC (optimum 30-37 ºC). Has no specific
requirement for vitamins.
Isolated from a sample of matured compost from the compost plant in Munster (Germany).
Susceptible to kanamycin, gentamicin, tetracycline and streptomycin.
  1. Wubbeler (J.H.), Lütke-Eversloh (T.), Van Trappen (S.), Vandamme (P.) And Steinbuchel (A.): Tetrathiobacter mimigardefordensis
    sp. nov., isolated from compost, a betaproteobacterium capable of utilizing the organic disulfide 3,3'-dithiodipropionic acid. Int. J.
    Syst. Evol. Microbiol., 2006, 56, 1305-1310.
  2. Gibello (A.), Vela (A.I.), Martin (M.), Barra-Caracciolo (A.), Grenni (P.) and Fernandez-Garayzabal (J.F.): Reclassification of the
    members of the genus Tetrathiobacter Ghosh et al. 2005 to the genus Advenella Coenye et al. 2005. Int. J. Syst. Evol. Microbiol.,
    2009, 59, 1914-1918
  3. Matsuoka (M.), Park (S.), An (S.Y.), Miyahara (M.), Kim (S.W.), Kamino (K.), Fushinobu (S.), Yokota (A.), Wakagi (T.) and Shoun (H.):
    Advenella faeciporci sp. nov., a nitrite-denitrifying bacterium isolated from nitrifying–denitrifying activated sludge collected from a
    laboratory-scale bioreactor treating piggery wastewater. Int. J. Syst. Evol. Microbiol., 2012, 62, 2986-2990.
Oxidative metabolism.

Positive results for acid and alkaline phosphatase, catalase, esterase (C4), esterase lipase (C8), leucine arylamidase, oxidase,
urease, naphthol-AS-BI-phosphohydrolase & valine arylamidase.
Can utilize: mercaptosuccinic acid, 3-mercaptopropionic acid, 2-mercaptopropionic acid, dibenzothiophene, benzothiophene, sodium
gluconate, glycerol, DL-lactate, D- and L-xylose, D-galactose, ribose, D-glucose, acetic acid, propionic acid, butyric acid, succinic acid,
L- and D-arabinose, adipic acid, citric acid, malic acid, Tween 80, D-galactose, D-mannose, formic acid, D-galactonic acid lactone,
D-galacturonic acid, D-gluconic acid, D-glucuronic acid, malonic acid, quinic acid, L-asparagine, L-aspartic acid, L-phenylalanine, D-
and L-fucose, gamma-aminobutyric acid, alpha-ketoglutaric acid, alpha-ketovaleric acid, glucose 6-phosphate, gamma-hydroxybutyric
acid, pyruvic acid methyl ester, succinic acid monomethyl ester, cis-aconitic acid, alpha- and beta-hydroxybutyric acid, itaconic acid,
alpha-ketobutyric acid, sebacic acid, succinic acid, bromosuccinic acid, succinamic acid, glucuronamide, alaninamide, D- and
L-alanine, L-alanyl glycine, L-glutamic acid, L-leucine, L-ornithine & L-proline.

Negative results for arginine dihydrolase, cystine arylamidase, esculin hydrolysis, alpha-fucosidase,alpha- and beta-galactosidase,
beta-glucuronidase, alpha- and beta-glucosidase, N-acetyl-beta-glucosaminidase, gelatin hydrolysis, indole production, lipase
(C14), alpha-mannosidase, protease, nitrate reduction, nitrite reduction to N
2, trypsin, alpha-chymotrypsin & glucose fermentation.
No utilization of: 3,3'-thiodipropionic acid, 4,4'-dithiodibutyric acid, thioglycolic acid,  thiodiglycolic acid, D-mannose, D-mannitol,
D-maltose, N-acetylglucosamine, capric acid, phenylacetic acid, glycyl L-aspartic acid, glycyl L-glutamic acid, L- and D-serine,
D-saccharic acid, hydroxy-L-proline, L-threonine, alpha-cyclodextrin, dextrin, Tween 40, N-acetyl-D-galactosamine,
N-acetyl-D-glucosamine, adonitol, D- and L-arabitol, cellobiose, i-erythritol, D-fructose, gentiobiose, myo-inositol, alpha-lactose,
lactulose, melibiose, methyl beta-D-glucoside, D-psicose, raffinose, L-rhamnose, D-sorbitol, sucrose, trehalose, turanose, xylitol,
D-glucosaminic acid, p-hydroxyphenylacetic acid, L-histidine, L-pyroglutamic acid, DL-carnitine, urocanic acid, inosine, uridine,
thymidine, phenylethylamine, putrescine, 2-aminoethanol, 2,3-butanediol, DL-alpha-glycerol phosphate, glucose 1-phosphate,  
turanose, tagatose, D-lyxose, xylitol, gentiobiose, glycogen & 2-ketogluconate.
(c) Costin Stoica
Culture media
Biochemical tests
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