Phylum Proteobacteria, Class Betaproteobacteria, Order Burkholderiales, Family Alcaligenaceae, Genus Advenella, Advenella
alkanexedens Wang et al. 2016.
Gram-negative coccoid cells, 0.6-0.8 µm. Nonmotile. Non-spore-forming.
Colonies on NA and TSA plates are 2.5-4.0 mm, slightly yellow to light brown in
colour, smooth and circular with regular margins after 36 hours of aerobic incubation.
The temperature range for growth is 4-52 ºC with an optimum at 30 to 35 ºC. The pH
range for growth is pH 5.1-9.7 with optimal growth at pH 8.5. Does not need NaCl for
growth, but tolerates up to 5.3 % (w/v) NaCl.
Isolated from a biogas slurry sample enriched with diesel oil.
- Huimin Wang, Shan Zhou, Yanwei Wang, Delong Kong, Xiang Guo, Jie Zhu, Weiwei Dong, Zhiyong Ruan, 2016. Advenella
alkanexedens sp. nov., an alkane-degrading bacterium isolated from biogas slurry samples. Int. J. Syst. Evol. Microbiol., 66, 906-
Biochemical description is based mostly on API ZYM, API 50CH, API 20NE and Biolog GN2 results.
Positive results for catalase, oxidase, esterase lipase, leucine arylamidase, valine arylamidase, naphthol-AS-BI-phosphohydrolase,
nitrate reduction, alkaline phosphatase, esterase, acid production from: D-fructose (weak), L-sorbose (weak), dulcitol (weak),
D-mannitol (weak), sorbitol (weak), arbutin, aesculin ferric citrate, salicin, D-cellobiose, D-maltose, D-lactose & D-melibiose (weak).
Can utilize: pyruvic acid methyl ester, succinic acid mono-methyl ester, acetic acid, cis-aconitic acid, citric acid, alpha- and
beta-hydroxybutyric acid, itaconic acid, alpha-ketobutyric acid, alpha-ketoglutaric acid, DL-lactic acid, propionic acid, sebacic acid,
succinic acid, bromosuccinic acid, succinamic acid, L-alaninamide, D- and L-alanine, L-glutamic acid, L-leucine, L-ornithine,
L-proline, adipic acid, malic acid, trisodium citrate, gamma-hydroxybutyric acid, alpha-ketovaleric acid, L-threonine,
gamma-aminobutyric acid, p-hydroxyphenlyacetic acid, D-mannitol (weak), D-maltose (weak), potassium gluconate (weak) &
Negative results for lipase, trypsin, alpha-chymotrypsin, alpha- and beta-galactosidase, beta-glucuronidase, alpha- and
beta-glucosidase, N-acetyl-beta-glucosaminidase, alpha-mannosidase, alpha-fucosidase, indole production, cystine arylamidase,
acid phosphatase, urease, acid production from: glycerol, D-ribose, DL-xylose & L-rhamnose.
No utilization of: D-mannose, N-acetylglucosamine, capric acid, Tween 80, alpha-D-glucose, D-saccharic acid, L-alanyl-glycine,
L-serine, glucuronamide & L-arabinose.
(c) Costin Stoica