Achromobacter spanius
Cultural characteristics
Biochemical characters
Phylum Proteobacteria, Class Betaproteobacteria, Order Burkholderiales, Family Alcaligenaceae, Genus Achromobacter,
Achromobacter spanius Coenye et al. 2003.
Gram-negative, small, coccoid rods, 1–2 μm long, occuring single, in pairs or in short
chains. Motility is variable.
On nutrient agar, colonies are flat or slightly convex with smooth margins and range
from white to light brown in colour. Can grow between 28 and 37 ºC and in 0-4.5%
NaCl. Does not grow on acetamide. Non-hemolytic. No growth on 10% lactose,
Tween 80 or oxidation–fermentation (OF) medium supplemented with glucose,
maltose, adonitol, fructose or xylose.
Isolated from blood.
  1. Coenye T., Vancanneyt M., Falsen E., Swings J., Vandamme P., 2003. Achromobacter insolitus sp. nov. and Achromobacter
    spanius sp. nov., from human clinical samples. Int J Syst Evol Microbiol 53(6):1819-1824 doi:10.1099/ijs.0.02698-0.
  2. Vandamme, P. A., Peeters, C., Inganas, E., Cnockaert, M., Houf, K., Spilker, T., Moore, E. R. B. and Lipuma, J. J. 2016. Taxonomic
    dissection of Achromobacter denitrificans Coenye et al. 2003 and proposal of Achromobacter agilis sp. nov., nom. rev.,
    Achromobacter pestifer sp. nov., nom. rev., Achromobacter kerstersii sp. nov. and Achromobacter deleyi sp. nov. Int. J. Syst. Evol.
    Microbiol., 66, 3708-3717.
Positive results for acid phosphatase, catalase, C4-esterase, leucine arylamidase, nitrate reduction & oxidase.
Assimilates isovalerate, glutarate, DL-glycerate, mesaconate, aconitate, L-norleucine, L-valine, L-cysteine, L-tryptophan,  tryptamine,  
gluconate, acetate, propionate, butyrate, isobutyrate, n-valerate, succinate, fumarate, adipate, pimelate, suberate, azelate, sebacate,
DL-lactate, DL-3-hydroxybutyrate, D- and L-malate, meso-tartrate, pyruvate, citraconate, itaconate, citrate, phenylacetate,
m-hydroxybenzoate, D- and L-alpha-alanine, L-leucine, L-isoleucine, L-serine, L-threonine, L-phenylalanine, L-histidine, L-aspartate,
L-glutamate, L-proline, DL-4-aminobutyrate, 2-aminobenzoate, 2-ketogluconate, L-tyrosine, beta-alanine, DL-3-aminobutyrate,
diaminobutane & tryptamine.

Negative results for alkaline phosphatase, amylase, arginine dihydrolase, cysteine arylamidase, DNase, esculin hydrolysis,
alpha-fucosidase, gelatinase, alpha- and beta-galactosidase, beta-glucuronidase, alpha- and beta-glucosidase,
N-acetyl-beta-glucosaminidase, H
2S production (TSI), indole production (TSI), lysine decarboxylase, C8-esterlipase, C14-lipase,
alpha-mannosidase, nitrite reduction, ornithine decarboxylase, trypsin, chymotrypsin, phosphoamidase, urease, valine arylamidase,

No assimilation of D-tartrate,  diaminobutane, glycerol, erythritol, D- and L-arabinose, ribose, D- and L-xylose, adonitol, methyl
beta-D-xyloside, galactose, D-glucose, D-fructose, D-mannose, L-sorbose, L-rhamnose, dulcitol, inositol, mannitol, sorbitol, methyl
alpha-D-mannoside, methyl alpha-D-glucoside, N-acetylglucosamine, amygdalin, arbutin, aesculin, salicin, cellobiose, maltose,
lactose, melibiose, sucrose, trehalose, inulin, D-melezitose, raffinose, starch, glycogen, xylitol, beta-gentiobiose, D-turanose,
D-lyxose, D-tagatose, D- and L-fucose, D- and L-arabitol, 5-ketogluconate, caprylate, oxalate, malonate, glycolate, levulinate,
o-hydroxybenzoate, D- and L-mandelate, phthalate, isophthalate, terephthalate, trigonelline, L-arginine, betaine, creatine,
DL-5-aminovalerate, 2-, 3- and 4-aminobenzoate, urea, acetamide, sarcosine, ethylamine, butylamine, amylamine, ethanolamine,
benzylamine, spermine, histamine, glucosamine, mesaconate, aconitate, n-caproate, heptanoate, pelargonate, caprate, benzoate,
p-hydroxybenzoate, glycine, L-ornithine, L-methionine, L-lysine, L-citrulline, DL-kynurenine.

Variable results for assimilation of isovalerate, glutarate, DL-glycerate, D- and L-tartrate, 2-oxoglutarate, DL-norvaline,
DL-2-aminobutyrate, L-cysteine,D- and L-tryptophan, .
(c) Costin Stoica
Culture media
Biochemical tests
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