Phylum Actinobacteria, Class Actinobacteria, Subclass Actibacteridae, Order Actynomycetales, Suborder Actinomycinae, Family
Actinomycetaceae, Genus Arcanobacterium, Arcanobacterium phocae Pascual Ramos, Foster and Collins, 1997.
Gram-positive coccobacilli and short rods, occurring singly, in pairs or in clusters;
short diphtheroid forms may occur. Non-motile. Non-spore-forming. Mycolic acids are
Colonies are white, tiny, low convex, after 24 h of incubation in anaerobic atmosphere,
on 5% sheep blood agar. Beta-haemolytic. Facultative anaerobic. Grows at 37 ºC.
Isolated from tissues and fluids of common seals (Phoca vitulina) and grey seals (Halichoerus grypus) in mixed cultures from
pneumonic and septicemic seals.
it is unclear but the strains have been recovered in mixed cultures from pneumonic and septicemic seals.
- Pascual Ramos C., Foster G. and Collins M. D., 1997. Phylogenetic analysis of the genus Actinomyces based on 16s rRNA gene
sequences: description of Arcanobacteriurn phocae sp. nov., Arcanobacterium bernardiae comb. nov., and Arcanobacterium
pyogenes comb. nov. Int. J. Syst. Bacteriol. Vol. 47, No. 1, 46-53.
Non-acid fast. Metabolism is strictly fermentative.
Most results are based on API 50 CH, API Coryne and API ZYM (bioMerieux) tests.
Positive results for acid and alkaline phosphatase, cystine arylamidase, esterase C4, esterase-lipase C8, alpha-glucosidase, alpha-
and beta-galactosidase, 5-ketogluconate, leucine arylamidase, pyrazinamidase, acid production from: D-fructose, galactose,
D-glucose, glycerol, glycogen, N-acetylglucosamine, lactose, maltose, D-ribose, starch, sucrose and D-turanose.
Negative results for alpha-chymotrypsin, esculin hydrolysis, alpha-fucosidase, gelatin hydrolysis, beta-glucuronidase,
beta-glucosidase, N-acetyl-beta-glucosaminidase, 2-ketogluconate, lipase (C14), alpha-mannosidase, nitrate reduction, pyrrolidonyl
arylamidase, urease, valine arylamidase, acid production from: adonitol amygdalin, L-arabinose, D- and L-arabitol, arbutin, cellobiose,
dulcitol, erythritol, D- and L-fucose, gentibiose, inulin, D-lyxose, melibiose, raffinose, L-rhamnose, salicin, D-sorbitol, sorbose, xylitol
Variable results for catalase, trypsin, acid production from: gluconate, inositol, mannitol, mannose, melezitose, D-tagatose, trehalose &
(c) Costin Stoica