Actinomyces timonensis
Taxonomy
Morphology
Cultural characteristics
Biochemical characters
Ecology
Pathogenicity
References
Phylum Actinobacteria, Class Actinobacteria, Subclass Actibacteridae, Order Actynomycetales, Suborder Actinomycinae, Family
Actinomycetaceae, Genus Actinomyces,
Actinomyces timonensis Renvoise, Raoult and Roux 2010.

Species description is based on a single isolate.
Gram-positive straight rods, 1.0-3.2 / 0.3-0.5 µm.
Colonies are white, dry, pinpoint and embedded in the agar supplemented with 5%
sheep blood after 48 h anaerobic incubation at 37 ºC. Grow in trypticase soy broth
anaerobically, but also grew under microaerophilic conditions and in 5% CO
2, and
weakly (after 48 h) in the presence of air. Alpha-haemolytic after 48 h of incubation.
Growth range is 25-37 ºC (37 ºC optimum).
Isolated from a human clinical osteo-articular sample (sacroiliitis).
Susceptible to amoxicillin-clavulanic acid, cefotetan, imipenem, penicillin G and vancomycin.
Isolated from a human clinical osteo-articular sample (sacroiliitis).
  1. Renvoise A., Raoult D. and Roux V, 2010. Actinomyces timonensis sp. nov., isolated from a human clinical osteo-articular
    sample.  Int. J. Syst. Evol. Microbiol. Vol. 60, 1516-1521.
Description is based on API ZYM, API 50CH, API Rapid ID32A identification kits and MALDI-TOF MS protein analysis.

Positive results for aesculin, beta-glucosidase, leucine arylamidase, proline arylamidase, phenylalanine arylamidase, tyrosine
arylamidase, acid production from: D-arabitol (weak reaction), D-adonitol (weak reaction), arbutin (weak reaction), fructose, galactose,
D-glucose, glycogen, glycerol, inositol, lactose, maltose, mannitol, melezitose, melibiose, N-acetylglucosamine, ribose, sucrose,
salicin and trehalose.

Negative results for alkaline phosphatase, arginine dihydrolase, arginine arylamidase, catalase, alpha-chymotrypsin, cystine
arylamidase, esterase C4, ester lipase C8, lipase C14, alpha-fucosidase, D- and L-fucose, alpha- and beta-galactosidase,
beta-glucuronidase, N-acetyl-beta-glucosaminidase, indole production, nitrate reduction, alpha-mannosidase, oxidase, urease,
valine  arylamidase, acid production from: amygdalin, D-arabinose, L-arabitol, cellobiose, dulcitol, erythritol, gentibiose,
alpha-D-glucopyranoside, inulin, 2- and 5-ketogluconate, D-lyxose, potassium gluconate, rhamnose, sorbitol, L-sorbose, starch
(amidon), tagatose, turanose, xylitol, L- and D-xylose.

Variable results for alpha-glucosidase, acid production from: mannose and raffinose.
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