Phylum Actinobacteria, Class Actinobacteria, Subclass Actibacteridae, Order Actynomycetales, Suborder Actinomycinae, Family
Actinomycetaceae, Genus Actinomyces, Actinomyces naturae Rao, Rash, Nobre, da Costa, Rainey, Moe, 2012.
Gram-positive rods, 0.5 / 2.0-2.5 µm. Non-acid-fast. Non-spore-forming. Motile
(flagella were observed by TEM and the motility was demonstrated in semisolid agar).
Colonies are convex, white, opaque, smooth and 1-2 mm in diameter on PCA (Plate
Count Agar) after 4 days at 30 ºC in anaerobic conditions; in aerobic conditions,
colonies were observed after 4 weeks at 37 ºC.
Grows in anaerobic PYG (Peptone/Yeast Extract/Glucose) broth. Growth temperature:
20-43 ºC (optimally at 30-37 ºC). Grows in pH 4.5-9.0 (pH 6.5, optimum) and in 0-3.0%
Isolated from chlorinated solvent contaminated groundwater at Superfund Site, near Baton Rouge, LA (USA).
- Rao JU, Rash BA, Nobre MF, da Costa MS, Rainey FA, Moe WM, 2012. Actinomyces naturae sp. nov., the first Actinomyces sp.
isolated from a non-human or animal source. Antonie van Leeuwenhoek 101, 155-168.
Description is based on API Rapid ID32A, API Rapid ID32 STREP, API Coryne and API ZYM results.
Positive results for esterase C4 (weak reaction), alpha- and beta-galactosidase, alpha- and beta-glucosidase, leucine arylamidase,
pyrazinamidase, acid production from D-glucose, maltose, mannose, melezitose, sucrose and D-xylose.
Negative results for acid and alkaline phosphatase, arginine dihydrolase, catalase, cystine arylamidase, esculin hydrolysis, esterase
lipase C8, alpha-fucosidase, beta-glucuronidase, hippurate hydrolysis, indole production, alpha-mannosidase,
N-acetyl-beta-glucosaminidase, lipase C14, nitrate reduction, oxidase, pyrrolidonyl arylamidase, urease, trypsin, alpha-chymotrypsin,
valine arylamidase, Voges-Proskauer test, acid production from D-arabitol, glycogen, lactose, mannitol, melibiose and raffinose.
(c) Costin Stoica