Acinetobacter lwoffii
Cultural characteristics
Biochemical characters
Phylum Proteobacteria, Class Gammaproteobacteria, Order Pseudomonadales, Family Moraxellaceae, Genus Acinetobacter,
Acinetobacter lwoffii  
(Audureau 1940) Brisou and Prevot 1954.

Old synonyms:
Moraxella lwoffi (sic) Audureau 1940, Acinetobacter lwoffi (sic)
(Audureau 1940) Brisou and Prevot 1954.
Gram-negative rods (0.9-1.6–1.5 x 1.5–2.5 μm), becoming spherical in the stationary
phase of growth. Grouped in pairs or chains. Nonmotile but may display “twitching
motility”. Nonsporulated.
Colonies on tryptocasein soy agar are  circular, convex, smooth, and slightly opaque
with entire margins; colonies are 1.0–1.5 mm in diameter after 24 h and 3.0–4.0 mm
in diameter after 48 h at 30 ºC. Good growth occurs at 15–37 ºC. Good growth occurs
at 15 to 30 ºC; no growth at 41 ºC. Aerobic. Nonhemolytic. No growth factor is required.
Isolated from clinical samples, the environment, rarely from foods.
May be involved in nosocomial infections. It is recognized as normal flora of the skin, oropharynx and perineum of healthy individuals.
There are few reports of bacteremia associated with indwelling catheters in humans, particularly in immunocompromised patients,
and neonatal infections.
  1. Elliot Juni and Kjell Bovre. Order IX Pseudomonadales Orla-Jensen 1921, Family II Moraxellaceae Rossau, Van Landschoot,
    Gillis and De Ley 1991 In:  Bergey’s Manual of Systematic Bacteriology, Second edition,Vol two, part B, George M. Garrity (Editor-in-
    Chief), pp. 411-442.
  2. Philippe J. M. Bouvet and Patrick A. D. Grimont. Taxonomy of the Genus Acinetobacter with the Recognition of Acinetobacter
    baumannii sp. nov., Acinetobacter haemolyticus sp. nov., Acinetobacter johnsonii sp. nov., and Acinetobacter junii sp. nov. and
    Emended Descriptions of Acinetobacter calcoaceticus and Acinetobacter lwoffii. Int J Syst Bacteriol April 1986 36:228-240.
  3. Sofia Constantiniu, Angela Romaniuc, Luminiţa Smaranda Iancu, Raluca Filimon, Iuliana Taraşi. Cultural And Biochemical
    Characteristics Of Acinetobacter spp. Strains Isolated From Hospital Units. The Journal Of Preventive Medicine 2004; 12 (3-4): 35-
  4. Narongsak Nakwan, Jeerawan Wannaro, Narongwit Nakwan. Multidrug-resistant Acinetobacter lwoffii infection in neonatal
    intensive care units. Research and Reports in Neonatology January 2011 Volume 2011:1, 1 - 4.
  5. Yukimasa Nishimura, Takeshi Ino, and Hiroshi Iizuka. Acinetobacter radioresistens sp. nov. Isolated from Cotton and Soil. Int J
    Syst Bacteriol April 1988 38:209-211.
Positive results for catalase, utilization of: azelate and DL-lactate.

Negative results for oxidase, nitrates reduction, indole production, H
2S production, phenylalanine deaminase, amylase, DNase,
beta-galactosidase, gelatin hydrolysis, beta-xylosidase, gamma-glutamyltransferase, methyl red, Voges-Proskauer reaction,
utilization of: citrate (Simmons), glutarate, L-phenylalanine, malonate, L-histidine, Laspartate, L-leucine, histamine, p-alanine,
2,3-butanediol, trans-aconitate, L-arginine, D-arabinose, dulcitol, beta-D-(-)-fructose, glycerol, glycine, glycogene, glycolate,
myoinositol, 2-keto-gluconate, 5-keto-gluconate, inulin, alpha-lactose, maltose, D-mandelate, D-mannitol, D-(+)-mannose,
D-(+)-melezitose, D-(+)-raffinose, alpha-L-rhamnose, salicin,  D-sorbitol, starch, sucrose, D-(+)-trehalose, D-xylitol & L-xylose.
Acid is not produced from D-glucose.

Variable utilization of: phenylacetate, D-malate, L-tyrosine, ethanol, L-ornithine & DL-4-aminobutyrate.
(c) Costin Stoica
Culture media
Biochemical tests
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