Phylum Proteobacteria, Class Gammaproteobacteria, Order Pseudomonadales, Family Moraxellaceae, Genus Acinetobacter,
Acinetobacter johnsonii Bouvet and Grimont 1986.
Gram-negative rods (0.9-1.6–1.5 x 1.5–2.5 μm), becoming spherical in the stationary
phase of growth. Grouped in pairs or chains. Nonmotile but may display “twitching
Colonies on Tryptocasein soy agar are circular, convex, smooth, and slightly opaque
with entire margins; 1.0 to 1.5 mm in diameter after 24 h and 2.0 to 3.0 mm in
diameter after 48 h at 30 ºC. Good growth occurs at 15 to 30 ºC; no growth occurs at
37 ºC. Aerobic. Nonhemolytic. No growth factor is required.
Isolated from clinical specimens, activated sludge, foods, and eviscerated chickens.
Associated with outbreaks of hospital infection. May cause ropiness in milk.
- Elliot Juni and Kjell Bovre. Order IX Pseudomonadales Orla-Jensen 1921, Family II Moraxellaceae Rossau, Van Landschoot,
Gillis and De Ley 1991 In: Bergey’s Manual of Systematic Bacteriology, Second edition,Vol two, part B, George M. Garrity (Editor-in-
Chief), pp. 411-442.
- Philippe J. M. Bouvet and Patrick A. D. Grimont. Taxonomy of the Genus Acinetobacter with the Recognition of Acinetobacter
baumannii sp. nov., Acinetobacter haemolyticus sp. nov., Acinetobacter johnsonii sp. nov., and Acinetobacter junii sp. nov. and
Emended Descriptions of Acinetobacter calcoaceticus and Acinetobacter lwoffii. Int J Syst Bacteriol April 1986 36:228-240.
Positive results for catalase, utilization of: citrate, DL-lactate & ethanol.
Variable utilization of: malonate, D-malate, L-aspartate, L-tyrosine, 2,3-butanediol,
L-arginine, L-ornithine & DL-4-aminobutyrate.
Negative results for oxidase, nitrates reduction, indole production, H2S production, phenylalanine deaminase, amylase, DNase,
beta-galactosidase, gelatin hydrolysis, beta-xylosidase and gamma-glutamyltransferase, utilization of: glucose, glutarate,
L-phenylalanine, phenylacetate, L-histidine, azelate, L-leucine, histamine, beta-alanine & trans-aconitate.
Acid is not produced from D-glucose.
(c) Costin Stoica