Legend: + positive 90-100%, - negative 90-100%, [+] positive 75-89%, [-] negative 75-89%, d positive 25-74% of strains
Differential characters of hemolytic Acinetobacter species:
Phylum Proteobacteria, Class Gammaproteobacteria, Order Pseudomonadales, Family Moraxellaceae, Genus Acinetobacter,
Acinetobacter haemolyticus (ex Stenzel and Mannheim 1963) Bouvet and Grimont 1986.
Old synonym: Achromobacter haemolyticus Stenzel and Mannheim 1963.
Gram-negative rods (0.9-1.6–1.5 x 1.5–2.5 μm), becoming spherical in the stationary
phase of growth. Grouped in pairs or chains. Aerobic. Nonmotile but may display
“twitching motility”. Nonsporulated.
Colonies on Tryptocasein soy agar are circular, convex, smooth, and slightly opaque
with entire margins; 1.5 to 2.0 mm in diameter after 24 h and 3.5 to 4.0 mm in
diameter after 48 h at 30 ºC. Sometimes have a sticky consistency. Good growth
occurs at 15 to 37 ºC in 24h. No growth occurs at 41 ºC. On sheep and horse blood
agar colonies are surrounded by a clear zone which is limited by a dark red ring after
24 h at 37 ºC or after 48 h at 30 ºC. No growth factor is required.
Isolated from clinical specimens and the environment.
- Elliot Juni and Kjell Bovre. Order IX Pseudomonadales Orla-Jensen 1921, Family II Moraxellaceae Rossau, Van Landschoot,
Gillis and De Ley 1991 In: Bergey’s Manual of Systematic Bacteriology, Second edition,Vol two, part B, George M. Garrity (Editor-in-
Chief), pp. 411-442.
- Philippe J. M. Bouvet and Patrick A. D. Grimont. Taxonomy of the Genus Acinetobacter with the Recognition of Acinetobacter
baumannii sp. nov., Acinetobacter haemolyticus sp. nov., Acinetobacter johnsonii sp. nov., and Acinetobacter junii sp. nov. and
Emended Descriptions of Acinetobacter calcoaceticus and Acinetobacter lwoffii. Int J Syst Bacteriol April 1986 36:228-240.
- Alexandr Nemec, Martin Musílek, Martina Maixnerová, Thierry De Baere, Tanny J. K. van der Reijden, Mario Vaneechoutte, and
Lenie Dijkshoorn. Acinetobacter beijerinckii sp. nov. and Acinetobacter gyllenbergii sp. nov., haemolytic organisms isolated from
humans . Int J Syst Evol Microbiol January 2009 59:118-124.
Positive results for catalase, gelatin hydrolysis (most strains), utilization of: citrate (most strains), DL-4-aminobutyrate, L-histidine,
D-malate, L-leucine, ethanol & L-arginine.
Negative results for oxidase, nitrates reduction, indole production, H2S production, phenylalanine deaminase, amylase, DNase,
beta-galactosidase, gamma-glutamyltransferase, utilization of: adipate, DL-lactate, azelate, histamine, beta-alanine, benzoate,
malonate, 2,3-butanediol & L-ornithine.
Variable results for beta-xylosidase and acid production from glucose.
(c) Costin Stoica