Differential characters of hemolytic Acinetobacter species:
Legend: + positive 90-100%, - negative 90-100%, [+] positive 75-89%, [-] negative 75-89%, d positive 25-74% of strains
Phylum Proteobacteria, Class Gammaproteobacteria, Order Pseudomonadales, Family Moraxellaceae, Genus Acinetobacter,
Acinetobacter beijerinckii Nemec et al. 2009.
Gram-negative coccobacilli. Nonmotile. Nonsporulated.
Colonies on tryptone soya agar after 24 h incubation at 30 ºC are approximately 1.0-
2.0 mm in diameter, circular, convex, smooth and slightly opaque with entire margins.
Growth occurs at 25-37 ºC, but not at 41 ºC. Strictly aerobic. Hemolytic; produce large
zones of hemolysis after 24 h on Columbia agar supplemented with sheep or horse
Isolated from human (sputum, peritoneal dialysis fluid, gall, throat swab, perineal swab, faeces) and equine (airways) clinical
specimens, from a hospital environment and staff (skin), soil and water.
Undetermined. Some strains were isolated from clinical samples and hospital environment.
- Alexandr Nemec, Martin Musílek, Martina Maixnerova, Thierry De Baere, Tanny J. K. van der Reijden, Mario Vaneechoutte, and
Lenie Dijkshoorn. Acinetobacter beijerinckii sp. nov. and Acinetobacter gyllenbergii sp. nov., haemolytic organisms isolated from
humans . Int J Syst Evol Microbiol January 2009 59:118-124.
Positive results for catalase, utilization of: acetate, ethanol, citrate (Simmons’),
4-aminobutyrate, L-aspartate, L-histidine, L-Leucine (most strains), D-malate &
Negative results for oxidase, gelatin hydrolysis (with some exceptions), utilization of: DL-lactate, trans-aconitate, glutarate, azelate,
beta-alanine, histamine, L-phenylalanine, phenylacetate, levulinate, citraconate, 4-hydroxybenzoate, L-tartrate, L-ornithine, L-arginine,
D-glucose, D-gluconate, D-ribose, L-arabinose, 2,3-butanediol, benzoate, adipate, gentisate, tryptamine, trigonelline, tricarballylate or
putrescine. Acid is not produced from glucose.
(c) Costin Stoica